Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/19925
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Oxidative status and histological changes in sea bass larvae muscle in response to high dietary content of docosahexaenoic acid DHA
Authors: Betancor, Monica
Caballero, Maria Jose
Benitez-Santana, Tibiabin
Saleh, Reda
Roo, Javier
Atalah, Eyad
Izquierdo, Marisol S
Contact Email: m.b.betancor@stir.ac.uk
Keywords: docosahexaenoic acid
larvae
muscle dystrophy
oxidative stress
sea bass
ultrastructure
Issue Date: May-2013
Publisher: Wiley-Blackwell
Citation: Betancor M, Caballero MJ, Benitez-Santana T, Saleh R, Roo J, Atalah E & Izquierdo MS (2013) Oxidative status and histological changes in sea bass larvae muscle in response to high dietary content of docosahexaenoic acid DHA, Journal of Fish Diseases, 36 (5), pp. 453-465.
Abstract: In previous studies, we observed dystrophic alterations in muscle of 48-day-old sea bass fed imbalanced docosahexaenoic acid (DHA) and vitamin E diets. To understand the whole pathological process associated with oxidative stress, a histological study was performed by feeding 14-day-old sea bass larvae with microdiets containing different ratios of DHA/vitamin E (1/150, 5/150 and 5/300) for a period of 21 days. Larvae fed diet 1/150 showed no lesions in contrast to larvae fed diets 5/150 and 5/300 where the highest incidence of muscle lesions and thiobarbituric acid reactive substances (TBARS) content was observed. Semithin sections revealed focal lesions consisting of degenerated fibres with hypercontracted myofilaments and extensive sarcoplasm vacuolization affecting both red and white muscle. Ultrathin sections of degenerating muscle fibres showed diffuse dilatation of sarcoplasmic reticulum, disorganized myofilaments and autophagic vacuoles containing myelin figures and dense bodies. Additionally, some macrophages were observed among injured fibres as numerous satellite cells. Results from the study agree with those obtained from previous work, proving the pathological potential of free radicals in sea bass larvae musculature. Moreover, high vitamin E inclusion could not completely protect cell membranes from free radicals action.
Type: Journal Article
URI: http://hdl.handle.net/1893/19925
DOI Link: http://dx.doi.org/10.1111/j.1365-2761.2012.01447.x
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Aquaculture
Universidad de Las Palmas de Gran Canaria
Universidad de Las Palmas de Gran Canaria
Universidad de Las Palmas de Gran Canaria
Universidad de Las Palmas de Gran Canaria
Universidad de Las Palmas de Gran Canaria
Universidad de Las Palmas de Gran Canaria

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