Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/19601
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Comparative study of pineal clock gene and AANAT2 expression in relation to melatonin synthesis in Atlantic salmon (Salmo salar) and European seabass (Dicentrarchus labrax)
Authors: McStay, Elsbeth
Migaud, Herve
Vera, LM
Sanchez-Vazquez, F Javier
Davie, Andrew
Contact Email: luisa.veraandujar@stir.ac.uk
Keywords: Pineal gland
Clock genes
Melatonin
Aanat2
Atlantic salmon
European seabass
Issue Date: Mar-2014
Publisher: Elsevier
Citation: McStay E, Migaud H, Vera L, Sanchez-Vazquez FJ & Davie A (2014) Comparative study of pineal clock gene and AANAT2 expression in relation to melatonin synthesis in Atlantic salmon (Salmo salar) and European seabass (Dicentrarchus labrax), Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology, 169, pp. 77-89.
Abstract: The photoreceptive teleost pineal is considered to be essential to the generation, synchronisation and maintenance of biological rhythms, primarily via melatonin release. The role of internal (circadian clock) and external (light) signals controlling melatonin production in the fish pineal differs between species, yet the reasons underpinning this remain largely unknown. Whilst in salmonids, pineal melatonin is apparently regulated directly by light, in all other studied teleosts, rhythmic melatonin production persists endogenously under the regulation of clock gene expression. To better understand the role of clocks in teleost pineals, this study aimed to characterise the expression of selected clock genes in vitro under different photoperiodic conditions in comparison to in vivo in both Atlantic salmon (Salmo salar) and in European seabass (Dicentrarchus labrax) (in vitro 12L:12D), a species known to display endogenous rhythmic melatonin synthesis. Results revealed no rhythmic clock gene (Clock, Period 1 &2) expression in Atlantic salmon or European seabass (Clock and Period 1) pineal in vitro. However rhythmic expression of Cryptochrome 2 and Period 1 in the Atlantic salmon pineal was observed in vivo, which infers extra-pineal regulation of clocks in this species. No rhythmic arylalkylamine N-acetyltransferase 2 (Aanat2) expression was observed in the Atlantic salmon yet in the European seabass, circadian Aanat2 expression was observed. Subsequent in silico analysis of available Aanat2 genomic sequences reveals that Atlantic salmon Aanat2 promoter sequences do not contain similar regulatory architecture as present in European seabass, and previously described in other teleosts which alludes to a loss in functional connection in the pathway.
Type: Journal Article
URI: http://hdl.handle.net/1893/19601
DOI Link: http://dx.doi.org/10.1016/j.cbpa.2013.12.011
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Aquaculture
Aquaculture
Aquaculture
University of Murcia
Aquaculture

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