Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/17371
Appears in Collections:Biological and Environmental Sciences Journal Articles
Peer Review Status: Refereed
Title: Characterization of microsatellite loci in Erysimum mediohispanicum (Brassicaceae) and cross-amplification in related species
Authors: Munoz-Pajares, A Jesus
Herrador, M Belen
Abdelaziz, Mohamed Mohamed
Pico, F Xavier
Sharbel, Timothy F
Gomez, Jose M
Perfectti, Francisco
Contact Email: ma38@stir.ac.uk
Keywords: Brassicaceae
cross-amplifi cation
Erysimum mediohispanicum
microsatellites.
Issue Date: Oct-2011
Citation: Munoz-Pajares AJ, Herrador MB, Abdelaziz Mohamed M, Pico FX, Sharbel TF, Gomez JM & Perfectti F (2011) Characterization of microsatellite loci in Erysimum mediohispanicum (Brassicaceae) and cross-amplification in related species, American Journal of Botany, 98 (10), pp. e287-e289.
Abstract: • Premise of the study : We have developed and optimized microsatellite loci from a genomic library of Erysimum mediohispanicum. Microsatellites were also tested for cross-amplifi cation in 31 other Erysimum species. • Methods and Results : A total of 10 microsatellite loci were successfully amplifi ed. They were polymorphic for 81 E. mediohispanicum individuals from two locations in Sierra Nevada (southeastern Spain), which showed similar patterns of genetic diversity. On average, microsatellites had 8.6 alleles per locus and an expected heterozygosity of 0.69. Only one locus signifi cantly departed from Hardy – Weinberg equilibrium in both locations. Most of the markers successfully amplifi ed in other Erysimum species. • Conclusions : The genetic attributes of microsatellite loci will allow their application to population genetic studies in Erysimum , such as genetic differentiation and structure, gene fl ow, pollinator-mediated speciation, and hybridization studies. 
DOI Link: http://dx.doi.org/10.3732/ajb.1100181
Rights: Publisher policy allows this work to be made available in this repository. Published in American Journal of Botany, October 2011 vol. 98 no. 10 e287-e289 by American Journal of Botany. The original publication is available at: http://www.amjbot.org/content/98/10/e287.short



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