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Appears in Collections:Biological and Environmental Sciences Journal Articles
Peer Review Status: Refereed
Title: Abundance, Distribution, and Activity of Fe(II)-Oxidizing and Fe(III)-Reducing Microorganisms in Hypersaline Sediments of Lake Kasin, Southern Russia
Authors: Emmerich, Maren
Bhansali, Ankita
Losekann-Behrens, Tina
Schröder, Christian
Kappler, Andreas
Behrens, Sebastian
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Issue Date: Jun-2012
Publisher: American Society for Microbiology
Citation: Emmerich M, Bhansali A, Losekann-Behrens T, Schröder C, Kappler A & Behrens S (2012) Abundance, Distribution, and Activity of Fe(II)-Oxidizing and Fe(III)-Reducing Microorganisms in Hypersaline Sediments of Lake Kasin, Southern Russia, Applied and Environmental Microbiology, 78 (12), pp. 4386-4399.
Abstract: The extreme osmotic conditions prevailing in hypersaline environments result in decreasing metabolic diversity with increasing salinity. Various microbial metabolisms have been shown to occur even at high salinity, including photosynthesis as well as sulfate and nitrate reduction. However, information about anaerobic microbial iron metabolism in hypersaline environments is scarce. We studied the phylogenetic diversity, distribution, and metabolic activity of iron(II)-oxidizing and iron(III)-reducing Bacteria and Archaea in pH-neutral, iron-rich salt lake sediments (Lake Kasin, southern Russia; salinity, 348.6 g liter-1) using a combination of culture-dependent and -independent techniques. 16S rRNA gene clone libraries for Bacteria and Archaea revealed a microbial community composition typical for hypersaline sediments. Most-probable-number counts confirmed the presence of 4.26×102 to 8.32×103 iron(II)-oxidizing Bacteria and 4.16×102 to 2.13×10 3 iron(III)-reducing microorganisms per gram dry sediment. Microbial iron(III) reduction was detected in the presence of 5 M NaCl, extending the natural habitat boundaries for this important microbial process. Quantitative real-time PCR showed that 16S rRNA gene copy numbers of total Bacteria, total Archaea, and species dominating the iron(III)-reducing enrichment cultures (relatives of Halobaculum gomorrense, Desulfosporosinus lacus, and members of the Bacilli) were highest in an iron oxide-rich sediment layer. Combined with the presented geochemical and mineralogical data, our findings suggest the presence of an active microbial iron cycle at salt concentrations close to the solubility limit of NaCl.
Type: Journal Article
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Rights: Publisher policy allows this work to be made available in this repository. Published in Applied and Environmental Microbiology June 2012 vol. 78 no. 12 pp.4386-4399 by the American Society for Microbiology. The original publication is available at:
Affiliation: University of Tuebingen (Eberhard Karls)
University of Tuebingen (Eberhard Karls)
University of Tuebingen (Eberhard Karls)
Biological and Environmental Sciences
University of Tuebingen (Eberhard Karls)
University of Tuebingen (Eberhard Karls)

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