Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/15939
Appears in Collections:Psychology Journal Articles
Peer Review Status: Refereed
Title: Functional connectivity between the thalamus and postsubiculum: Analysis of evoked responses elicited by stimulation of the laterodorsal thalamic nucleus in anesthetized rats
Authors: Shires, Kate L
Hawthorne, James P
Hope, Alexander M J
Dudchenko, Paul
Wood, Emma R
Martin, Steven J
Contact Email: p.a.dudchenko@stir.ac.uk
Keywords: dorsal presubiculum
synaptic transmission
synaptic plasticity
long-term depression
urethane
Issue Date: Jul-2013
Publisher: Wiley-Blackwell
Citation: Shires KL, Hawthorne JP, Hope AMJ, Dudchenko P, Wood ER & Martin SJ (2013) Functional connectivity between the thalamus and postsubiculum: Analysis of evoked responses elicited by stimulation of the laterodorsal thalamic nucleus in anesthetized rats, Hippocampus, 23 (7), pp. 559-569.
Abstract: The laterodorsal nucleus (LDN) of the thalamus provides a prominent afferent projection to the postsubiculum (dorsal presubiculum). To characterize synaptic transmission in this pathway, we placed stimulating electrodes in the LDN and recorded fEPSPs elicited in the postsubiculum of urethane-anesthetized rats. LDN stimulation elicited a source-sink dipole between the deep and superficial layers of the postsubiculum, respectively, consistent with anatomical evidence for the termination of thalamic afferents in the superficial layers of the structure, and the existence of deep layer neurons with apical dendrites extending into these layers. Postsubicular fEPSPs were typically 0.5-1.0 mV in amplitude, with a peak latency of approximately 6 ms. Consistent with anatomical observations, the short onset latency of fEPSPs elicited by LDN stimulation, and their ability to follow a 60-Hz train of stimulation, indicate that the projection is monosynaptic. Paired-pulse stimulation revealed pronounced paired-pulse depression that was maximal at 100 ms, suggesting that initial release probabilities are high at LDN-postsubiculum synapses, in common with many neocortical pathways. A conventional tetanus protocol that yields LTP in hippocampal pathways had no effect on postsubicular fEPSPs, but long-term depression could be induced by 60-Hz stimulation. Drug infusion studies revealed that synaptic transmission in the LDN-postsubiculum projection is predominantly AMPA-receptor mediated. Rats were implanted with indwelling infusion cannulae targeting the postsubiculum, and, after a recovery period, were anaesthetized withurethane, and implanted with stimulating and recording electrodes. Infusion of CNQX almost completely abolished postsubicular fEPSPs, whereas D-AP5 had little effect. However, 60-Hz LTD was blocked by D-AP5 infusion, revealing that this form of synaptic plasticity is NMDA-receptor dependent.
Type: Journal Article
URI: http://hdl.handle.net/1893/15939
DOI Link: http://dx.doi.org/10.1002/hipo.22114
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: University of Edinburgh
University of Edinburgh
University of Edinburgh
Psychology
University of Edinburgh
University of Edinburgh

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