Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/15701
Appears in Collections:Biological and Environmental Sciences Journal Articles
Peer Review Status: Refereed
Title: The assessment of insemination success in yellow dung flies using competitive PCR
Authors: Bussiere, Luc
Demont, Marco
Pemberton, Andrew J
Hall, Matthew D
Ward, Paul I
Contact Email: luc.bussiere@stir.ac.uk
Keywords: cryptic choice
DNA quantification
mate choice
paternity
sperm competition
sperm selection
Issue Date: Mar-2010
Publisher: Wiley-Blackwell
Citation: Bussiere L, Demont M, Pemberton AJ, Hall MD & Ward PI (2010) The assessment of insemination success in yellow dung flies using competitive PCR, Molecular Ecology Resources, 10 (2), pp. 292-303.
Abstract: In spite of considerable interest in postcopulatory sexual selection, separating the effects of sperm competition from cryptic female choice remains difficult because mechanisms underlying postcopulatory processes are poorly understood. One methodological challenge is to quantify insemination success for individual males within the sperm stores of multiply mated females to discover how insemination translates into eventual paternity. Any proposed method must be applicable in organisms without extensive DNA sequence information (which include the majority of model species for sexual selection). Here, we describe the development and application of microsatellite competitive-multiplex-PCR for quantifying relative contributions to a small number of sperm in storage. We studied how DNA template characteristics affect PCR amplification of known concentrations of mixed DNA and generated regressions for correcting observations of allelic signal strength based on such characteristics. We used these methods to examine patterns of sperm storage in twice-mated female yellow dung flies, Scathophaga stercoraria. We confirm previous findings supporting sperm displacement and demonstrate that average paternity for the last mate accords with the mean proportion of sperm stored. We further find consistent skew in storage across spermathecae, with more last male sperm stored in the singlet spermatheca on one side of the body than in the doublet on the opposite side. We also show that the time between copulations may be important for effectively sorting sperm. Finally, we demonstrate that male size may influence the opportunity for sperm choice, suggesting future work to disentangle the roles of male competition and cryptic female choice.
Type: Journal Article
URI: http://hdl.handle.net/1893/15701
DOI Link: http://dx.doi.org/10.1111/j.1755-0998.2009.02754.x
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Biological and Environmental Sciences
University of Munich
University of Zurich
University of New South Wales
University of Zurich

Files in This Item:
File Description SizeFormat 
The assessment of insemination success in yellow dung flies.pdf259.61 kBAdobe PDFUnder Embargo until 31/12/2999     Request a copy

Note: If any of the files in this item are currently embargoed, you can request a copy directly from the author by clicking the padlock icon above. However, this facility is dependant on the depositor still being contactable at their original email address.

This item is protected by original copyright



Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.