Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/15458
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Cellular and molecular immune responses of the sea bass (Dicentrarchus labrax) experimentally infected with betanodavirus
Authors: Scapigliati, Giuseppe
Buonocore, Francesco
Randelli, Elisa
Casani, Daniela
Meloni, Sabrina
Zarletti, Gianpaolo
Tiberi, Mario
Pietretti, D
Boschi, Illenia
Manchado, Manuel
Martin-Antonio, Beatriz
Jimenez-Cantizano, Rosa M
Bovo, Giuseppe
Borghesan, Fabio
Lorenzen, Niels
Einer-Jensen, Katja
Adams, Alexandra
Thompson, Kimberly
Alonso, Carlos
Bejar, Julia
Cano, Irene
Borrego, Juan J
Alvarez, M Carmen
Contact Email: alexandra.adams@stir.ac.uk
Keywords: Sea bass Dicentrarchus labrax
Betanodavirus VER
PCR array
Lymphocytes
Antibody
Anti-viral immune defence
Issue Date: Feb-2010
Publisher: Elsevier
Citation: Scapigliati G, Buonocore F, Randelli E, Casani D, Meloni S, Zarletti G, Tiberi M, Pietretti D, Boschi I, Manchado M, Martin-Antonio B, Jimenez-Cantizano RM, Bovo G, Borghesan F, Lorenzen N, Einer-Jensen K, Adams A, Thompson K, Alonso C, Bejar J, Cano I, Borrego JJ & Alvarez MC (2010) Cellular and molecular immune responses of the sea bass (Dicentrarchus labrax) experimentally infected with betanodavirus, Fish and Shellfish Immunology, 28 (2), pp. 303-311.
Abstract: Naïve sea bass juveniles (38.4 ± 4.5 g) were intramuscularly infected with a sublethal dose of betanodavirus isolate 378/I03, followed after 43 days by a similar boosting. This infection resulted in an overall mortality of 7.6%. At various intervals, sampling of fish tissues was performed to investigate: i) B and T lymphocyte content in organs and tissues; ii), proliferation of leucocytes re-stimulated in vitro with inactivated virus; iii) presence of serum antibody specific for betanodavirus; iv) expression of genes coding for the following immunoregulatory molecules involved in innate and acquired responses: type I IFN, Mx, IL-1, Cox-2; IL-10, TGF-β, TCRβ, CD4, CD8α, IgM, by using a quantitative PCR array system developed for sea bass. The obtained results showed a detectable increase of T cells and B cells in PBL during betanodavirus infection. Furthermore, leucocytes obtained from blood, head kidney, and gills showed a detectable "in vitro" increase in viability upon addition of inactivated viral particles, as determined by measuring intracellular ATP concentration. ELISA analysis of sera showed that exposure to nodavirus induced a low, but specific antibody titer measured 43 days after infection, despite the presence of measurable levels of natural antibody. Finally, a strong upregulation of genes coding for type I IFN, Mx, and IgM was identified after both infection and boosting. Interestingly, an upregulation of Cox-2 until boosting, and of TGF-β and IL-10 after boosting was also observed, while the other tested genes did not show any significant variations with respect to mock-treated fish. Overall, our work represents a first comprehensive analysis of cellular and molecular immune parameters in a fish species exposed to a pathogenic virus.
Type: Journal Article
URI: http://hdl.handle.net/1893/15458
DOI Link: http://dx.doi.org/10.1016/j.fsi.2009.11.008
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: University of Tuscia
University of Tuscia
University of Tuscia
University of Tuscia
University of Tuscia
University of Tuscia
University of Tuscia
University of Tuscia
University of Tuscia
Centro IFAPA, Spain
Centro IFAPA, Spain
Centro IFAPA, Spain
Istituto Zooprofilattico Sperimentale delle Venezie, Italy
Istituto Zooprofilattico Sperimentale delle Venezie, Italy
Danish Institute for Food and Veterinary Research
Danish Institute for Fisheries Research
Aquaculture
Aquaculture
University of Malaga
University of Malaga
University of Malaga
University of Malaga
University of Malaga

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