Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/15458
Full metadata record
DC FieldValueLanguage
dc.contributor.authorScapigliati, Giuseppeen_UK
dc.contributor.authorBuonocore, Francescoen_UK
dc.contributor.authorRandelli, Elisaen_UK
dc.contributor.authorCasani, Danielaen_UK
dc.contributor.authorMeloni, Sabrinaen_UK
dc.contributor.authorZarletti, Gianpaoloen_UK
dc.contributor.authorTiberi, Marioen_UK
dc.contributor.authorPietretti, Den_UK
dc.contributor.authorBoschi, Illeniaen_UK
dc.contributor.authorManchado, Manuelen_UK
dc.contributor.authorMartin-Antonio, Beatrizen_UK
dc.contributor.authorJimenez-Cantizano, Rosa Men_UK
dc.contributor.authorBovo, Giuseppeen_UK
dc.contributor.authorBorghesan, Fabioen_UK
dc.contributor.authorLorenzen, Nielsen_UK
dc.contributor.authorEiner-Jensen, Katjaen_UK
dc.contributor.authorAdams, Alexandraen_UK
dc.contributor.authorThompson, Kimberlyen_UK
dc.contributor.authorAlonso, Carlosen_UK
dc.contributor.authorBejar, Juliaen_UK
dc.contributor.authorCano, Ireneen_UK
dc.contributor.authorBorrego, Juan Jen_UK
dc.contributor.authorAlvarez, M Carmenen_UK
dc.date.accessioned2013-06-14T23:18:19Z-
dc.date.available2013-06-14T23:18:19Zen_UK
dc.date.issued2010-02en_UK
dc.identifier.urihttp://hdl.handle.net/1893/15458-
dc.description.abstractNaïve sea bass juveniles (38.4 ± 4.5 g) were intramuscularly infected with a sublethal dose of betanodavirus isolate 378/I03, followed after 43 days by a similar boosting. This infection resulted in an overall mortality of 7.6%. At various intervals, sampling of fish tissues was performed to investigate: i) B and T lymphocyte content in organs and tissues; ii), proliferation of leucocytes re-stimulated in vitro with inactivated virus; iii) presence of serum antibody specific for betanodavirus; iv) expression of genes coding for the following immunoregulatory molecules involved in innate and acquired responses: type I IFN, Mx, IL-1, Cox-2; IL-10, TGF-β, TCRβ, CD4, CD8α, IgM, by using a quantitative PCR array system developed for sea bass. The obtained results showed a detectable increase of T cells and B cells in PBL during betanodavirus infection. Furthermore, leucocytes obtained from blood, head kidney, and gills showed a detectable "in vitro" increase in viability upon addition of inactivated viral particles, as determined by measuring intracellular ATP concentration. ELISA analysis of sera showed that exposure to nodavirus induced a low, but specific antibody titer measured 43 days after infection, despite the presence of measurable levels of natural antibody. Finally, a strong upregulation of genes coding for type I IFN, Mx, and IgM was identified after both infection and boosting. Interestingly, an upregulation of Cox-2 until boosting, and of TGF-β and IL-10 after boosting was also observed, while the other tested genes did not show any significant variations with respect to mock-treated fish. Overall, our work represents a first comprehensive analysis of cellular and molecular immune parameters in a fish species exposed to a pathogenic virus.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationScapigliati G, Buonocore F, Randelli E, Casani D, Meloni S, Zarletti G, Tiberi M, Pietretti D, Boschi I, Manchado M, Martin-Antonio B, Jimenez-Cantizano RM, Bovo G, Borghesan F, Lorenzen N, Einer-Jensen K, Adams A, Thompson K, Alonso C, Bejar J, Cano I, Borrego JJ & Alvarez MC (2010) Cellular and molecular immune responses of the sea bass (Dicentrarchus labrax) experimentally infected with betanodavirus. Fish and Shellfish Immunology, 28 (2), pp. 303-311. https://doi.org/10.1016/j.fsi.2009.11.008en_UK
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.rights.urihttp://www.rioxx.net/licenses/under-embargo-all-rights-reserveden_UK
dc.subjectSea bass Dicentrarchus labraxen_UK
dc.subjectBetanodavirus VERen_UK
dc.subjectPCR arrayen_UK
dc.subjectLymphocytesen_UK
dc.subjectAntibodyen_UK
dc.subjectAnti-viral immune defenceen_UK
dc.subjectSea basses Diseasesen_UK
dc.subjectEuropean seabassen_UK
dc.titleCellular and molecular immune responses of the sea bass (Dicentrarchus labrax) experimentally infected with betanodavirusen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2999-12-29en_UK
dc.rights.embargoreason[Fish and Shellfish Immunology - Sea bass.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.identifier.doi10.1016/j.fsi.2009.11.008en_UK
dc.citation.jtitleFish and Shellfish Immunologyen_UK
dc.citation.issn1050-4648en_UK
dc.citation.volume28en_UK
dc.citation.issue2en_UK
dc.citation.spage303en_UK
dc.citation.epage311en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emailalexandra.adams@stir.ac.uken_UK
dc.contributor.affiliationUniversity of Tusciaen_UK
dc.contributor.affiliationUniversity of Tusciaen_UK
dc.contributor.affiliationUniversity of Tusciaen_UK
dc.contributor.affiliationUniversity of Tusciaen_UK
dc.contributor.affiliationUniversity of Tusciaen_UK
dc.contributor.affiliationUniversity of Tusciaen_UK
dc.contributor.affiliationUniversity of Tusciaen_UK
dc.contributor.affiliationUniversity of Tusciaen_UK
dc.contributor.affiliationUniversity of Tusciaen_UK
dc.contributor.affiliationCentro IFAPA, Spainen_UK
dc.contributor.affiliationCentro IFAPA, Spainen_UK
dc.contributor.affiliationCentro IFAPA, Spainen_UK
dc.contributor.affiliationIstituto Zooprofilattico Sperimentale delle Venezie, Italyen_UK
dc.contributor.affiliationIstituto Zooprofilattico Sperimentale delle Venezie, Italyen_UK
dc.contributor.affiliationDanish Institute for Food and Veterinary Researchen_UK
dc.contributor.affiliationDanish Institute for Fisheries Researchen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Malagaen_UK
dc.contributor.affiliationUniversity of Malagaen_UK
dc.contributor.affiliationUniversity of Malagaen_UK
dc.contributor.affiliationUniversity of Malagaen_UK
dc.contributor.affiliationUniversity of Malagaen_UK
dc.identifier.isiWOS:000274721300008en_UK
dc.identifier.scopusid2-s2.0-74749098931en_UK
dc.identifier.wtid698487en_UK
dcterms.dateAccepted2010-02-28en_UK
dc.date.filedepositdate2013-06-12en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorScapigliati, Giuseppe|en_UK
local.rioxx.authorBuonocore, Francesco|en_UK
local.rioxx.authorRandelli, Elisa|en_UK
local.rioxx.authorCasani, Daniela|en_UK
local.rioxx.authorMeloni, Sabrina|en_UK
local.rioxx.authorZarletti, Gianpaolo|en_UK
local.rioxx.authorTiberi, Mario|en_UK
local.rioxx.authorPietretti, D|en_UK
local.rioxx.authorBoschi, Illenia|en_UK
local.rioxx.authorManchado, Manuel|en_UK
local.rioxx.authorMartin-Antonio, Beatriz|en_UK
local.rioxx.authorJimenez-Cantizano, Rosa M|en_UK
local.rioxx.authorBovo, Giuseppe|en_UK
local.rioxx.authorBorghesan, Fabio|en_UK
local.rioxx.authorLorenzen, Niels|en_UK
local.rioxx.authorEiner-Jensen, Katja|en_UK
local.rioxx.authorAdams, Alexandra|en_UK
local.rioxx.authorThompson, Kimberly|en_UK
local.rioxx.authorAlonso, Carlos|en_UK
local.rioxx.authorBejar, Julia|en_UK
local.rioxx.authorCano, Irene|en_UK
local.rioxx.authorBorrego, Juan J|en_UK
local.rioxx.authorAlvarez, M Carmen|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2999-12-29en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||en_UK
local.rioxx.filenameFish and Shellfish Immunology - Sea bass.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source1050-4648en_UK
Appears in Collections:Aquaculture Journal Articles

Files in This Item:
File Description SizeFormat 
Fish and Shellfish Immunology - Sea bass.pdfFulltext - Published Version519.54 kBAdobe PDFUnder Embargo until 2999-12-29    Request a copy


This item is protected by original copyright



Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

The metadata of the records in the Repository are available under the CC0 public domain dedication: No Rights Reserved https://creativecommons.org/publicdomain/zero/1.0/

If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.