Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/11469
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Development of a cDNA microarray for the measurement of gene expression in the sheep scab mite Psoroptes ovis
Authors: Burgess, Stewart
Downing, Alison
Watkins, Craig
Marr, Edward
Nisbet, Alasdair
Kenyon, Fiona
McNair, Carol
Huntley, John
Contact Email: c.m.mcnair@stir.ac.uk
Keywords: Psoroptes
sheep
microarray
gene
expression
Issue Date: 8-Feb-2012
Publisher: BioMed Central Ltd
Citation: Burgess S, Downing A, Watkins C, Marr E, Nisbet A, Kenyon F, McNair C & Huntley J (2012) Development of a cDNA microarray for the measurement of gene expression in the sheep scab mite Psoroptes ovis, Parasites and Vectors, 5 (30).
Abstract: Background: Sheep scab is caused by the ectoparasitic mite Psoroptes ovis which initiates a profound cutaneous inflammatory response, leading to the development of the skin lesions which are characteristic of the disease. Existing control strategies rely upon injectable endectocides and acaricidal dips but concerns over residues, eco-toxicity and the development of acaricide resistance limit the sustainability of this approach. In order to identify alternative means of disease control, a deeper understanding of both the parasite and its interaction with the host are required. Methods: Herein we describe the development and utilisation of an annotated P. ovis cDNA microarray containing 3,456 elements for the measurement of gene expression in this economically important ectoparasite. The array consists of 981 P. ovis EST sequences printed in triplicate along with 513 control elements. Array performance was validated through the analysis of gene expression differences between fed and starved P. ovis mites. Results: Sequences represented on the array include homologues of major house dust mite allergens and tick salivary proteins, along with factors potentially involved in mite reproduction and xenobiotic metabolism. In order to validate the performance of this unique resource under biological conditions we used the array to analyse gene expression differences between fed and starved P. ovis mites. These analyses identified a number of house dust mite allergen homologues up-regulated in fed mites and P. ovis transcripts involved in stress responses, autophagy and chemosensory perception up-regulated in starved mites. Conclusion: The P. ovis cDNA microarray described here has been shown to be both robust and reproducible and will enable future studies to analyse gene expression in this important ectoparasite.
Type: Journal Article
URI: http://hdl.handle.net/1893/11469
DOI Link: http://dx.doi.org/10.1186/1756-3305-5-30
Rights: © 2012 Burgess et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Affiliation: The Moredun Research Institute
Roslin Institute
The Moredun Research Institute
The Moredun Research Institute
The Moredun Research Institute
The Moredun Research Institute
Aquaculture
The Moredun Research Institute

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