Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/11469
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dc.contributor.authorBurgess, Stewarten_UK
dc.contributor.authorDowning, Alisonen_UK
dc.contributor.authorWatkins, Craigen_UK
dc.contributor.authorMarr, Edwarden_UK
dc.contributor.authorNisbet, Alasdairen_UK
dc.contributor.authorKenyon, Fionaen_UK
dc.contributor.authorMcNair, Carolen_UK
dc.contributor.authorHuntley, Johnen_UK
dc.date.accessioned2013-03-20T17:08:46Z-
dc.date.available2013-03-20T17:08:46Z-
dc.date.issued2012-02-08en_UK
dc.identifier.urihttp://hdl.handle.net/1893/11469-
dc.description.abstractBackground: Sheep scab is caused by the ectoparasitic mite Psoroptes ovis which initiates a profound cutaneous inflammatory response, leading to the development of the skin lesions which are characteristic of the disease. Existing control strategies rely upon injectable endectocides and acaricidal dips but concerns over residues, eco-toxicity and the development of acaricide resistance limit the sustainability of this approach. In order to identify alternative means of disease control, a deeper understanding of both the parasite and its interaction with the host are required. Methods: Herein we describe the development and utilisation of an annotated P. ovis cDNA microarray containing 3,456 elements for the measurement of gene expression in this economically important ectoparasite. The array consists of 981 P. ovis EST sequences printed in triplicate along with 513 control elements. Array performance was validated through the analysis of gene expression differences between fed and starved P. ovis mites. Results: Sequences represented on the array include homologues of major house dust mite allergens and tick salivary proteins, along with factors potentially involved in mite reproduction and xenobiotic metabolism. In order to validate the performance of this unique resource under biological conditions we used the array to analyse gene expression differences between fed and starved P. ovis mites. These analyses identified a number of house dust mite allergen homologues up-regulated in fed mites and P. ovis transcripts involved in stress responses, autophagy and chemosensory perception up-regulated in starved mites. Conclusion: The P. ovis cDNA microarray described here has been shown to be both robust and reproducible and will enable future studies to analyse gene expression in this important ectoparasite.en_UK
dc.language.isoenen_UK
dc.publisherBioMed Central Ltden_UK
dc.relationBurgess S, Downing A, Watkins C, Marr E, Nisbet A, Kenyon F, McNair C & Huntley J (2012) Development of a cDNA microarray for the measurement of gene expression in the sheep scab mite Psoroptes ovis. Parasites and Vectors, 5 (30). https://doi.org/10.1186/1756-3305-5-30en_UK
dc.rights© 2012 Burgess et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en_UK
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/en_UK
dc.subjectPsoroptesen_UK
dc.subjectsheepen_UK
dc.subjectmicroarrayen_UK
dc.subjectgeneen_UK
dc.subjectexpressionen_UK
dc.titleDevelopment of a cDNA microarray for the measurement of gene expression in the sheep scab mite Psoroptes ovisen_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1186/1756-3305-5-30en_UK
dc.citation.jtitleParasites and Vectorsen_UK
dc.citation.issn1756-3305en_UK
dc.citation.volume5en_UK
dc.citation.issue30en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emailc.m.mcnair@stir.ac.uken_UK
dc.contributor.affiliationThe Moredun Research Instituteen_UK
dc.contributor.affiliationRoslin Instituteen_UK
dc.contributor.affiliationThe Moredun Research Instituteen_UK
dc.contributor.affiliationThe Moredun Research Instituteen_UK
dc.contributor.affiliationThe Moredun Research Instituteen_UK
dc.contributor.affiliationThe Moredun Research Instituteen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationThe Moredun Research Instituteen_UK
dc.identifier.isiWOS:000301662700002en_UK
dc.identifier.scopusid2-s2.0-84856604527en_UK
dc.identifier.wtid758115en_UK
dcterms.dateAccepted2012-02-08en_UK
dc.date.filedepositdate2013-03-04en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorBurgess, Stewart|en_UK
local.rioxx.authorDowning, Alison|en_UK
local.rioxx.authorWatkins, Craig|en_UK
local.rioxx.authorMarr, Edward|en_UK
local.rioxx.authorNisbet, Alasdair|en_UK
local.rioxx.authorKenyon, Fiona|en_UK
local.rioxx.authorMcNair, Carol|en_UK
local.rioxx.authorHuntley, John|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2013-03-04en_UK
local.rioxx.licencehttp://creativecommons.org/licenses/by/2.0/|2013-03-04|en_UK
local.rioxx.filenamePsoroptes microarray.pdfen_UK
local.rioxx.filecount1en_UK
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