%PDF-1.6
%
1 0 obj
<>
endobj
2 0 obj
<>stream
Joanna Wilson, Iveta Matejusova, Rebecca E. McIntosh, Stefano Carboni, Michaël Bekaert
New diagnostic SNP molecular markers for the Mytilus species complex
www.plosone.org
www.plosone.org
endstream
endobj
3 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 14 0 R/Annots 15 0 R/Contents 16 0 R/TrimBox[0 0 612 792]>>
endobj
15 0 obj
[17 0 R 18 0 R 19 0 R 20 0 R 21 0 R 22 0 R 23 0 R 24 0 R 25 0 R 26 0 R 27 0 R 28 0 R 29 0 R 30 0 R 31 0 R 32 0 R 33 0 R 34 0 R 35 0 R 36 0 R 37 0 R 38 0 R 39 0 R 40 0 R 41 0 R 42 0 R 43 0 R]
endobj
17 0 obj
<>/Border[0 0 0]/Dest(Rpone.0200654.ref015)>>
endobj
18 0 obj
<>/Border[0 0 0]/Dest(Rpone.0200654.ref017)>>
endobj
19 0 obj
<>/Border[0 0 0]/Dest(Rpone.0200654.ref018)>>
endobj
20 0 obj
<>/Border[0 0 0]/Dest(Rpone.0200654.ref019)>>
endobj
21 0 obj
<>/Border[0 0 0]/Dest(Rpone.0200654.ref021)>>
endobj
22 0 obj
<>/Border[0 0 0]/Dest(Rpone.0200654.ref022)>>
endobj
23 0 obj
<>/Border[0 0 0]/Dest(Rpone.0200654.ref023)>>
endobj
24 0 obj
<>/Border[0 0 0]/Dest(Rpone.0200654.ref024)>>
endobj
25 0 obj
<>/Border[0 0 0]/Dest(Rpone.0200654.ref026)>>
endobj
26 0 obj
<>/Border[0 0 0]/Dest(Rpone.0200654.ref010)>>
endobj
27 0 obj
<>/Border[0 0 0]/Dest(Rpone.0200654.ref014)>>
endobj
28 0 obj
<>/Border[0 0 0]/Dest(Rpone.0200654.ref023)>>
endobj
29 0 obj
<>/Border[0 0 0]/Dest(Rpone.0200654.ref027)>>
endobj
30 0 obj
<>/Border[0 0 0]/Dest(Rpone.0200654.ref028)>>
endobj
31 0 obj
<>/Border[0 0 0]/Dest(Rpone.0200654.ref029)>>
endobj
32 0 obj
<>/Border[0 0 0]/Dest(Rpone.0200654.ref030)>>
endobj
33 0 obj
<>/Border[0 0 0]/Dest(Rpone.0200654.ref017)>>
endobj
34 0 obj
<>/Border[0 0 0]/Dest(Rpone.0200654.ref031)>>
endobj
35 0 obj
<>/Border[0 0 0]/Dest(Rpone.0200654.ref035)>>
endobj
36 0 obj
<>/Border[0 0 0]/Dest(Rpone.0200654.ref012)>>
endobj
37 0 obj
<>/Border[0 0 0]/Dest(Rpone.0200654.ref036)>>
endobj
38 0 obj
<>/Border[0 0 0]/Dest(Rpone.0200654.ref003)>>
endobj
39 0 obj
<>/Border[0 0 0]/Dest(Rpone.0200654.ref010)>>
endobj
40 0 obj
<>/Border[0 0 0]/Dest(Rpone.0200654.ref011)>>
endobj
41 0 obj
<>/Border[0 0 0]/Dest(Rpone.0200654.ref037)>>
endobj
42 0 obj
<>/Border[0 0 0]/Dest(Rpone.0200654.ref038)>>
endobj
43 0 obj
<>/Border[0 0 0]/A 44 0 R>>
endobj
44 0 obj
<>
endobj
16 0 obj
[45 0 R 46 0 R 47 0 R 48 0 R 49 0 R 50 0 R 51 0 R 52 0 R 53 0 R 54 0 R 55 0 R 56 0 R 57 0 R 58 0 R 59 0 R 60 0 R 61 0 R 62 0 R 63 0 R 64 0 R 65 0 R 66 0 R 67 0 R 68 0 R 69 0 R 70 0 R 71 0 R]
endobj
45 0 obj
<>stream
q
0.83 0.64 0.02 0 k
203.4142 667.3323 m
212.9386 667.3323 l
h
f*
218.0976 667.3323 m
227.622 667.3323 l
h
f*
357.3354 641.3102 m
366.8598 641.3102 l
h
f*
550.9417 589.3228 m
560.4094 589.3228 l
h
f*
200.0126 576.3402 m
209.537 576.3402 l
h
f*
551.5654 576.3402 m
561.0898 576.3402 l
h
f*
327.2882 563.3008 m
336.8126 563.3008 l
h
f*
427.8614 550.3181 m
437.3858 550.3181 l
h
f*
442.5449 550.3181 m
452.0693 550.3181 l
h
f*
394.8094 498.3307 m
404.2772 498.3307 l
h
f*
406.5449 498.3307 m
416.0693 498.3307 l
h
f*
418.337 498.3307 m
427.8614 498.3307 l
h
f*
430.0724 498.3307 m
439.5969 498.3307 l
h
f*
449.4614 472.3087 m
458.9858 472.3087 l
h
f*
555.9307 446.3433 m
565.4551 446.3433 l
h
f*
557.4047 420.3213 m
566.9291 420.3213 l
h
f*
292.422 381.3165 m
301.9465 381.3165 l
h
f*
258.5764 355.3512 m
268.1008 355.3512 l
h
f*
273.2598 355.3512 m
282.7843 355.3512 l
h
f*
239.6409 342.3118 m
249.1087 342.3118 l
h
f*
251.3764 342.3118 m
260.9008 342.3118 l
h
f*
203.4142 107.9433 m
208.1764 107.9433 l
h
f*
210.3874 107.9433 m
219.9118 107.9433 l
h
f*
222.1795 107.9433 m
231.7039 107.9433 l
h
f*
233.9717 107.9433 m
243.4394 107.9433 l
h
f*
245.7071 107.9433 m
255.2315 107.9433 l
h
f*
0 g
1 j
1 J
0 w
10 0 0 10 200.0125 707.4141 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
[()47()-15()7()4()12()-4()]TJ
/F7 1 Tf
3.1804 0 Td
(species)Tj
2.999 0 Td
(complex)Tj
3.6227 0 Td
(is)Tj
0.8504 0 Td
(therefore)Tj
3.8494 0 Td
(important)Tj
4.286 0 Td
(for)Tj
1.3833 0 Td
(the)Tj
1.4683 0 Td
(management)Tj
5.4368 0 Td
(of)Tj
1.0148 0 Td
(a)Tj
0.652 0 Td
(potentially)Tj
4.4617 0 Td
(economi-)Tj
-33.2048 -1.3039 Td
(cally)Tj
2.0239 0 Td
(damaging)Tj
4.1782 0 Td
(species.)Tj
-5.0059 -1.2982 Td
(Hybridisation)Tj
5.7996 0 Td
(and)Tj
1.7291 0 Td
(introgression)Tj
5.5219 0 Td
(between)Tj
3.5263 0 Td
(species)Tj
2.999 0 Td
(are)Tj
1.4457 0 Td
(common)Tj
3.8437 0 Td
(evolutionary)Tj
5.2611 0 Td
(phenomena)Tj
-31.3226 -1.2983 Td
([)Tj
0.83 0.64 0.02 0 k
(15)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(17)Tj
0 g
(].)Tj
3.5432 0 Td
(Introgression)Tj
5.5899 0 Td
(arises)Tj
2.4378 0 Td
(from)Tj
2.2053 0 Td
(repeated)Tj
3.6397 0 Td
(backcrossing)Tj
5.3858 0 Td
(with)Tj
1.9956 0 Td
(fertile)Tj
2.5398 0 Td
(hybrids,)Tj
3.4639 0 Td
(allowing)Tj
3.617 0 Td
(stable)Tj
-34.418 -1.2983 Td
(integration)Tj
4.6487 0 Td
(of)Tj
1.0148 0 Td
(genomic)Tj
3.6737 0 Td
(material)Tj
3.515 0 Td
(of)Tj
1.0147 0 Td
(one)Tj
1.6952 0 Td
(species)Tj
2.999 0 Td
(into)Tj
1.8425 0 Td
(the)Tj
1.4683 0 Td
(genome)Tj
3.4073 0 Td
(of)Tj
1.0148 0 Td
(another)Tj
3.3335 0 Td
(species)Tj
2.9933 0 Td
(without)Tj
3.3336 0 Td
(a)Tj
-35.9544 -1.3039 Td
(significant)Tj
4.388 0 Td
(deleterious)Tj
4.5977 0 Td
(effect)Tj
2.3641 0 Td
(on)Tj
1.2756 0 Td
(fitness)Tj
2.7666 0 Td
([)Tj
0.83 0.64 0.02 0 k
(18)Tj
0 g
(].)Tj
2.0749 0 Td
(Accurate)Tj
3.8098 0 Td
(identification)Tj
5.5842 0 Td
(of)Tj
1.0148 0 Td
(species)Tj
2.9933 0 Td
(\(including)Tj
4.3824 0 Td
(cryp-)Tj
-35.2514 -1.2982 Td
(tic)Tj
1.2075 0 Td
(or)Tj
1.0998 0 Td
(complex)Tj
3.6227 0 Td
(species\))Tj
3.3335 0 Td
(is)Tj
0.8504 0 Td
(important)Tj
4.2803 0 Td
(from)Tj
2.2053 0 Td
(commercial,)Tj
5.1817 0 Td
(conservation)Tj
5.3631 0 Td
(and)Tj
1.7292 0 Td
(research)Tj
3.5603 0 Td
(viewpoints)Tj
-32.4338 -1.2983 Td
(and)Tj
1.7291 0 Td
(can)Tj
1.627 0 Td
(be)Tj
1.1509 0 Td
(significantly)Tj
5.0853 0 Td
(impaired)Tj
3.8495 0 Td
(when)Tj
2.4037 0 Td
(hybridisation)Tj
5.5616 0 Td
(or)Tj
1.0941 0 Td
(introgression)Tj
5.5162 0 Td
(occur.)Tj
-26.8212 -1.3039 Td
(There)Tj
2.5795 0 Td
(are)Tj
1.4513 0 Td
(some)Tj
2.3301 0 Td
(distinguishing)Tj
5.9187 0 Td
(morphological)Tj
6.0604 0 Td
(features)Tj
3.3449 0 Td
(that)Tj
1.7801 0 Td
(could)Tj
2.4491 0 Td
(be)Tj
1.1509 0 Td
(employed)Tj
4.1385 0 Td
(for)Tj
1.389 0 Td
(marine)Tj
-33.7887 -1.2983 Td
(mussel)Tj
2.9593 0 Td
(species)Tj
2.9934 0 Td
(\()Tj
/F8 1 Tf
[()47()-15()7()4()12()-4()]TJ
/F7 1 Tf
3.5262 0 Td
(spp.\))Tj
2.1827 0 Td
(identification,)Tj
5.811 0 Td
(such)Tj
2.0579 0 Td
(as)Tj
1.0148 0 Td
(shell)Tj
2.024 0 Td
(colour,)Tj
3.0273 0 Td
(shape,)Tj
2.7099 0 Td
(texture)Tj
3.0274 0 Td
(and)Tj
1.7291 0 Td
(size)Tj
1.6895 0 Td
([)Tj
0.83 0.64 0.02 0 k
(19)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
-34.7525 -1.2983 Td
(21)Tj
0 g
(].)Tj
1.7347 0 Td
(However,)Tj
4.0762 0 Td
(a)Tj
0.6577 0 Td
(range)Tj
2.4604 0 Td
(of)Tj
1.0148 0 Td
(biotic)Tj
2.4888 0 Td
(and)Tj
1.7292 0 Td
(abiotic)Tj
2.9253 0 Td
(factors,)Tj
3.1294 0 Td
(including)Tj
4.0422 0 Td
(hydrodynamic)Tj
6.0831 0 Td
(conditions)Tj
4.473 0 Td
([)Tj
0.83 0.64 0.02 0 k
(22)Tj
0 g
(],)Tj
-34.8148 -1.3039 Td
(water)Tj
2.4321 0 Td
(temperature)Tj
5.125 0 Td
(and)Tj
1.7291 0 Td
(salinity)Tj
3.1011 0 Td
([)Tj
0.83 0.64 0.02 0 k
(23)Tj
0 g
(],)Tj
2.0749 0 Td
(can)Tj
1.6271 0 Td
(affect)Tj
2.3754 0 Td
(these)Tj
2.2507 0 Td
(features)Tj
3.3505 0 Td
(making)Tj
3.2485 0 Td
(individuals)Tj
4.6488 0 Td
(often)Tj
2.2847 0 Td
(mor-)Tj
-34.2479 -1.2982 Td
(phologically)Tj
5.0626 0 Td
(similar,)Tj
3.2088 0 Td
(especially)Tj
4.0195 0 Td
(in)Tj
1.0318 0 Td
(sympatric)Tj
4.1669 0 Td
(populations)Tj
4.9549 0 Td
([)Tj
0.83 0.64 0.02 0 k
(24)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(26)Tj
0 g
(].)Tj
-21.2483 -1.2983 Td
(The)Tj
1.7858 0 Td
(development)Tj
5.4084 0 Td
(of)Tj
1.0148 0 Td
(diagnostic)Tj
4.32 0 Td
(markers)Tj
3.4979 0 Td
(has)Tj
1.5421 0 Td
(been)Tj
2.1203 0 Td
(a)Tj
0.6576 0 Td
(long-standing)Tj
5.7883 0 Td
(interest)Tj
3.2088 0 Td
(of)Tj
1.0148 0 Td
(population)Tj
-31.555 -1.3039 Td
(geneticists)Tj
4.3539 0 Td
(as)Tj
1.0148 0 Td
(it)Tj
0.7881 0 Td
(allows)Tj
2.6985 0 Td
(clarification)Tj
4.9946 0 Td
(of)Tj
1.0148 0 Td
(taxonomic)Tj
4.4901 0 Td
(uncertainties.)Tj
5.6352 0 Td
(The)Tj
1.7858 0 Td
(first)Tj
1.8029 0 Td
(tools)Tj
2.1429 0 Td
(used)Tj
2.058 0 Td
(to)Tj
1.0261 0 Td
(distin-)Tj
-33.8057 -1.2983 Td
(guish)Tj
2.3697 0 Td
(between)Tj
3.5263 0 Td
(mussel)Tj
2.9593 0 Td
(species)Tj
2.9991 0 Td
(were)Tj
2.1203 0 Td
(allozymes.)Tj
4.3483 0 Td
(Numerous)Tj
4.4957 0 Td
(allozyme)Tj
3.7644 0 Td
(markers)Tj
3.4979 0 Td
(have)Tj
2.058 0 Td
(been)Tj
2.1259 0 Td
(devel-)Tj
-34.2649 -1.2982 Td
(oped)Tj
2.194 0 Td
(and)Tj
1.7291 0 Td
(used)Tj
2.0636 0 Td
(for)Tj
/F8 1 Tf
1.3833 0 Td
[()47()-15()7()4()12()-4()]TJ
/F7 1 Tf
3.1861 0 Td
(species)Tj
2.9934 0 Td
(identification)Tj
5.5899 0 Td
([)Tj
0.83 0.64 0.02 0 k
(10)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(14)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(23)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(27)Tj
0 g
(].)Tj
5.6069 0 Td
(Nonetheless,)Tj
5.3007 0 Td
(low)Tj
1.6554 0 Td
(as)Tj
1.0148 0 Td
(well)Tj
1.8199 0 Td
(as)Tj
1.0148 0 Td
(high)Tj
-35.5519 -1.2983 Td
(variation)Tj
3.7984 0 Td
(between)Tj
3.5319 0 Td
(individual)Tj
4.286 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
/F8 1 Tf
1.4683 0 Td
[()47()-15()7()4()12()-4()]TJ
/F7 1 Tf
3.1861 0 Td
(complex)Tj
3.6227 0 Td
(render)Tj
2.8856 0 Td
(the)Tj
1.4684 0 Td
(technique)Tj
4.1669 0 Td
(only)Tj
1.9785 0 Td
(marginally)Tj
-31.4076 -1.3039 Td
(more)Tj
2.3414 0 Td
(useful)Tj
2.5908 0 Td
(than)Tj
2.0296 0 Td
(the)Tj
1.4683 0 Td
(identification)Tj
5.5899 0 Td
(by)Tj
1.1792 0 Td
(morphological)Tj
6.0605 0 Td
(features)Tj
3.3448 0 Td
([)Tj
0.83 0.64 0.02 0 k
(28)Tj
0 g
(].)Tj
2.0806 0 Td
(Over)Tj
2.2167 0 Td
(the)Tj
1.4684 0 Td
(past)Tj
1.8311 0 Td
(three)Tj
-32.2013 -1.2983 Td
(decades,)Tj
3.5602 0 Td
(a)Tj
0.6463 0 Td
(range)Tj
2.4605 0 Td
(of)Tj
1.0091 0 Td
(species)Tj
2.9934 0 Td
(diagnostic)Tj
4.3143 0 Td
(markers)Tj
3.4923 0 Td
(have)Tj
2.0522 0 Td
(been)Tj
2.1147 0 Td
(developed)Tj
4.2689 0 Td
(for)Tj
1.3833 0 Td
(single)Tj
2.5228 0 Td
(locus)Tj
2.2791 0 Td
(genotyping)Tj
-33.0971 -1.2983 Td
(of)Tj
/F8 1 Tf
1.0147 0 Td
[()47()-15()7()4()12()-4()]TJ
/F7 1 Tf
3.1805 0 Td
(species)Tj
2.999 0 Td
(of)Tj
1.0148 0 Td
(which)Tj
2.6532 0 Td
(the)Tj
1.4684 0 Td
(most)Tj
2.2053 0 Td
(routinely)Tj
3.8665 0 Td
(used)Tj
2.0579 0 Td
(is)Tj
0.8447 0 Td
(the)Tj
1.474 0 Td
(nuclear)Tj
3.1918 0 Td
(DNA)Tj
2.3754 0 Td
(marker)Tj
3.1408 0 Td
(Me15/16)Tj
3.7644 0 Td
([)Tj
0.83 0.64 0.02 0 k
(29)Tj
0 g
(].)Tj
-35.2514 -1.3039 Td
(Genotyping)Tj
4.9662 0 Td
(with)Tj
1.9956 0 Td
(the)Tj
1.4683 0 Td
(Me15/16)Tj
3.7701 0 Td
(is)Tj
0.8447 0 Td
(favoured)Tj
3.7587 0 Td
(due)Tj
1.6951 0 Td
(to)Tj
1.0262 0 Td
(its)Tj
1.1452 0 Td
(simple)Tj
2.8573 0 Td
(methodology)Tj
5.5048 0 Td
(of)Tj
1.0148 0 Td
(PCR)Tj
2.0636 0 Td
(amplification)Tj
-32.1106 -1.2982 Td
(and)Tj
1.7291 0 Td
(identification)Tj
5.5842 0 Td
(of)Tj
1.0148 0 Td
(a)Tj
0.6576 0 Td
(size-specific)Tj
5.006 0 Td
(gene)Tj
2.0806 0 Td
(fragments)Tj
4.2293 0 Td
(unique)Tj
3.0217 0 Td
(for)Tj
1.3833 0 Td
(each)Tj
2.0296 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
/F8 1 Tf
1.4683 0 Td
[()47()-15()7()4()12()-4()]TJ
/F7 1 Tf
3.1861 0 Td
(species)Tj
2.9991 0 Td
([)Tj
0.83 0.64 0.02 0 k
(30)Tj
0 g
(].)Tj
-35.4045 -1.2983 Td
(Single)Tj
2.6475 0 Td
(locus)Tj
2.2847 0 Td
(genotyping)Tj
4.7112 0 Td
(delivers)Tj
3.2995 0 Td
(more)Tj
2.3414 0 Td
(accurate)Tj
3.5546 0 Td
(identification)Tj
5.5842 0 Td
(of)Tj
/F8 1 Tf
1.0148 0 Td
[()47()-15()7()4()12()-4()]TJ
/F7 1 Tf
3.1861 0 Td
(species.)Tj
3.2202 0 Td
(than)Tj
2.0296 0 Td
(studying)Tj
-33.8738 -1.3039 Td
(morphology)Tj
5.142 0 Td
(or)Tj
1.0998 0 Td
(allozymes,)Tj
4.3483 0 Td
(but)Tj
1.5534 0 Td
(has)Tj
1.542 0 Td
(limited)Tj
3.0728 0 Td
(potential)Tj
3.7587 0 Td
(for)Tj
1.3833 0 Td
(analysing)Tj
3.9798 0 Td
(patterns)Tj
3.4753 0 Td
(of)Tj
1.0148 0 Td
(hybridisation)Tj
5.5558 0 Td
(or)Tj
-35.926 -1.2983 Td
(genome)Tj
3.3958 0 Td
(introgression)Tj
5.5049 0 Td
([)Tj
0.83 0.64 0.02 0 k
(17)Tj
0 g
(].)Tj
2.0693 0 Td
(Multilocus)Tj
4.507 0 Td
(genotyping,)Tj
4.9323 0 Td
(using)Tj
2.3811 0 Td
(genome)Tj
3.3958 0 Td
(wide)Tj
2.1147 0 Td
(panels)Tj
2.7382 0 Td
(of)Tj
1.0092 0 Td
(single)Tj
2.5171 0 Td
(nucleo-)Tj
-34.5654 -1.2983 Td
(tide)Tj
1.7347 0 Td
(polymorphism)Tj
6.1342 0 Td
(\(SNP\))Tj
2.6759 0 Td
(markers)Tj
3.4922 0 Td
(by)Tj
1.1849 0 Td
(comparison,)Tj
5.1987 0 Td
(allows)Tj
2.6986 0 Td
(for)Tj
1.3889 0 Td
(a)Tj
0.652 0 Td
(far)Tj
1.3153 0 Td
(better)Tj
2.5398 0 Td
(understanding)Tj
6.0718 0 Td
(of)Tj
-35.087 -1.2982 Td
(introgression)Tj
5.5161 0 Td
([)Tj
0.83 0.64 0.02 0 k
(31)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(35)Tj
0 g
(].)Tj
3.5433 0 Td
(There)Tj
2.5795 0 Td
(are)Tj
1.4457 0 Td
(only)Tj
1.9786 0 Td
(few)Tj
1.6157 0 Td
(studies)Tj
2.9877 0 Td
(on)Tj
1.2756 0 Td
(multilocus)Tj
4.4447 0 Td
(genotyping)Tj
4.7111 0 Td
(in)Tj
1.0375 0 Td
(Mytilus)Tj
3.2712 0 Td
(species)Tj
-34.4067 -1.3039 Td
(complex)Tj
3.6226 0 Td
([)Tj
0.83 0.64 0.02 0 k
(12)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(36)Tj
0 g
(],)Tj
3.2542 0 Td
(which)Tj
2.6475 0 Td
(are)Tj
1.4513 0 Td
(however)Tj
3.6113 0 Td
(limited)Tj
3.0671 0 Td
(to)Tj
1.0261 0 Td
(small)Tj
2.3244 0 Td
(number)Tj
3.4186 0 Td
(of)Tj
1.0148 0 Td
(markers)Tj
3.4979 0 Td
(used)Tj
2.0636 0 Td
(to)Tj
1.0205 0 Td
(resolve)Tj
3.0104 0 Td
(popu-)Tj
-35.0303 -1.2983 Td
(lation)Tj
2.5171 0 Td
(structures)Tj
4.1839 0 Td
(based)Tj
2.4775 0 Td
(on)Tj
1.2756 0 Td
(allele)Tj
2.2393 0 Td
(frequencies.)Tj
-11.4972 -1.2983 Td
(In)Tj
1.1111 0 Td
(this)Tj
1.6725 0 Td
(study,)Tj
2.6192 0 Td
(we)Tj
1.3266 0 Td
(have)Tj
2.0579 0 Td
(employed)Tj
4.1386 0 Td
(an)Tj
1.2018 0 Td
(easy)Tj
1.8936 0 Td
(and)Tj
1.7348 0 Td
(rapid)Tj
/F8 1 Tf
2.3357 0 Td
[( )4()]TJ
1.1055 0 Td
[()6()12()3()]TJ
/F7 1 Tf
2.1146 0 Td
(SNP)Tj
1.99 0 Td
(discovery)Tj
4.0138 0 Td
(method)Tj
3.3222 0 Td
(to)Tj
-33.8341 -1.3039 Td
(develop)Tj
3.3165 0 Td
(genome-wide)Tj
5.6636 0 Td
(species-specific)Tj
6.3155 0 Td
(markers)Tj
3.4979 0 Td
(to)Tj
1.0262 0 Td
(genotype)Tj
3.8607 0 Td
(the)Tj
/F8 1 Tf
1.4684 0 Td
[()47()-15()7()4()12()-4()]TJ
/F7 1 Tf
3.1804 0 Td
(species)Tj
2.9991 0 Td
(complex,)Tj
-31.3283 -1.2982 Td
(which)Tj
2.6475 0 Td
(also)Tj
1.7688 0 Td
(identify)Tj
3.2938 0 Td
(hybrids)Tj
3.2372 0 Td
(and)Tj
1.7291 0 Td
(introgressed)Tj
5.1477 0 Td
(individuals)Tj
4.6488 0 Td
(in)Tj
1.0375 0 Td
(field)Tj
1.9729 0 Td
(populations.)Tj
5.176 0 Td
(More)Tj
2.4151 0 Td
(accurate)Tj
-33.0744 -1.2983 Td
(characterisation)Tj
6.6103 0 Td
(of)Tj
/F8 1 Tf
1.0148 0 Td
[()47()-15()7()4()12()-4()]TJ
/F7 1 Tf
3.1805 0 Td
(specie)Tj
2.6362 0 Td
(populations')Tj
5.1703 0 Td
(structure)Tj
3.8211 0 Td
(will)Tj
1.6611 0 Td
(aid)Tj
1.4513 0 Td
(improved)Tj
4.1102 0 Td
(conservation)Tj
5.3631 0 Td
(and)Tj
-35.0189 -1.3039 Td
(aquaculture)Tj
4.9379 0 Td
(management)Tj
5.4368 0 Td
(strategies.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 239.1874 cm
BT
/F3 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Materials)Tj
4.5071 0 Td
(and)Tj
1.989 0 Td
(methods)Tj
/F15 1 Tf
-6.4961 -1.4976 Td
(Ethics)Tj
2.7779 0 Td
(statement)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 204.2078 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Animal)Tj
3.2201 0 Td
(\(mussels\))Tj
3.9968 0 Td
(handling)Tj
3.7871 0 Td
(and)Tj
1.7291 0 Td
(collection)Tj
4.1102 0 Td
(was)Tj
1.7008 0 Td
(done)Tj
2.228 0 Td
(under)Tj
2.6192 0 Td
(Marine)Tj
3.1578 0 Td
(Science)Tj
3.2031 0 Td
(Scotland)Tj
3.6794 0 Td
(\(Scottish)Tj
-33.4316 -1.2982 Td
(Government\))Tj
5.6692 0 Td
(authority)Tj
3.9061 0 Td
(and)Tj
1.7291 0 Td
(following)Tj
3.9799 0 Td
(both)Tj
2.0636 0 Td
(Marine)Tj
3.1521 0 Td
(Science)Tj
3.2088 0 Td
(Scotland)Tj
3.6793 0 Td
(and)Tj
1.7291 0 Td
(University)Tj
4.4051 0 Td
(of)Tj
1.0148 0 Td
(Stirling)Tj
-34.5371 -1.3039 Td
(Ethical)Tj
2.9876 0 Td
(recommendations)Tj
7.5005 0 Td
(and)Tj
1.7291 0 Td
(guidance.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 151.9937 cm
BT
/F15 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Sample)Tj
3.2787 0 Td
(collection)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 134.9858 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Adult)Tj
2.5058 0 Td
(mussels)Tj
3.3165 0 Td
(\(at)Tj
1.2926 0 Td
(least)Tj
1.9842 0 Td
(40)Tj
1.1679 0 Td
(mm)Tj
1.8595 0 Td
(in)Tj
1.0375 0 Td
(length\))Tj
3.0727 0 Td
(were)Tj
2.1203 0 Td
(collected)Tj
3.7417 0 Td
(from)Tj
2.2053 0 Td
(regions)Tj
3.1635 0 Td
(where)Tj
2.6475 0 Td
(pure)Tj
/F8 1 Tf
2.0636 0 Td
[()47()-15()7()4()12()-4()]TJ
/F7 1 Tf
3.1805 0 Td
(spe-)Tj
-35.3591 -1.2982 Td
(cies)Tj
1.6951 0 Td
(were)Tj
2.1203 0 Td
(reported)Tj
3.6623 0 Td
(to)Tj
1.0261 0 Td
(occur,)Tj
2.6986 0 Td
(based)Tj
2.4718 0 Td
(on)Tj
1.2756 0 Td
(historical,)Tj
4.1442 0 Td
(genetic)Tj
3.0727 0 Td
(analysis)Tj
3.3279 0 Td
(or)Tj
1.0942 0 Td
(morphological)Tj
6.0604 0 Td
(evidence)Tj
-32.6492 -1.2983 Td
([)Tj
0.83 0.64 0.02 0 k
(3)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(10)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(11)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(37)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(38)Tj
0 g
(].)Tj
6.3042 0 Td
(Specimens)Tj
4.4843 0 Td
(of)Tj
/F8 1 Tf
1.0148 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
1.3153 0 Td
[()7( )12()4()12()-4()]TJ
/F7 1 Tf
2.4661 0 Td
(were)Tj
2.126 0 Td
(collected)Tj
3.736 0 Td
(from)Tj
2.2054 0 Td
(two)Tj
1.7121 0 Td
(shoreline)Tj
3.8948 0 Td
(locations)Tj
3.8154 0 Td
(in)Tj
1.0318 0 Td
(south-)Tj
-34.1062 -1.3039 Td
(west)Tj
1.9842 0 Td
(Scotland)Tj
3.685 0 Td
([Loch)Tj
2.5512 0 Td
(Ryan)Tj
2.279 0 Td
(\(LR\))Tj
2.0636 0 Td
(and)Tj
1.7291 0 Td
(Rascarrel)Tj
3.9062 0 Td
(Bay)Tj
1.6894 0 Td
(\(RB\)])Tj
2.4434 0 Td
(and)Tj
1.7292 0 Td
(one)Tj
1.6951 0 Td
(shoreline)Tj
3.9004 0 Td
(location)Tj
3.4469 0 Td
(in)Tj
1.0375 0 Td
(east)Tj
-34.1402 -1.2983 Td
(Scotland)Tj
3.6793 0 Td
([Montrose)Tj
4.4674 0 Td
(\(MON\)],)Tj
3.8437 0 Td
(the)Tj
1.4684 0 Td
(three)Tj
2.2677 0 Td
(sites)Tj
1.9275 0 Td
(have)Tj
2.0636 0 Td
(shellfish)Tj
3.4639 0 Td
(farming)Tj
3.4129 0 Td
(activities)Tj
3.7191 0 Td
(in)Tj
1.0318 0 Td
(the)Tj
1.474 0 Td
(vicinities;)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 107.1496 23.6976 re
W* n
q
107.0929 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 463.011 745.7952 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(SNP)Tj
2.2464 0 Td
(markers)Tj
3.7772 0 Td
(for)Tj
1.3677 0 Td
(the)Tj
/F6 1 Tf
1.5875 0 Td
(Mytilus)Tj
/F0 1 Tf
3.2811 0 Td
(spp.)Tj
ET
Q
q
1 j
1 J
0 w
36 48.0002 m
576 48.0002 l
576 47.5002 l
36 47.5002 l
f*
0.83 0.64 0.02 0 k
81.5811 34.9228 m
237.8835 34.9228 l
h
f*
0 g
7.9999 0 0 7.9999 36 36 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(PLOS)Tj
2.8559 0 Td
(ONE)Tj
2.3669 0 Td
(|)Tj
0.83 0.64 0.02 0 k
0.4748 0 Td
(https://doi.or)Tj
5.4142 0 Td
(g/10.137)Tj
3.8198 0 Td
(1/journal.po)Tj
5.1236 0 Td
(ne.02006)Tj
4.089 0 Td
(54)Tj
0 g
2.0906 0 Td
(July)Tj
1.9701 0 Td
(12,)Tj
1.5874 0 Td
(2018)Tj
35.3483 0 Td
(2)Tj
0.7654 0 Td
(/)Tj
0.496 0 Td
(13)Tj
/F12 1 Tf
-0.025 Tc
-66.402 84.0904 Td
(Scotland)Tj
3.4582 0 Td
(Science.)Tj
3.3237 0 Td
(Publication)Tj
4.3158 0 Td
(fees)Tj
1.7362 0 Td
(were)Tj
2.0126 0 Td
(coved)Tj
2.4308 0 Td
(by)Tj
-17.2773 -1.3748 Td
(the)Tj
1.3677 0 Td
(University)Tj
3.9189 0 Td
(of)Tj
0.9496 0 Td
(Stirling)Tj
2.8347 0 Td
(to)Tj
0.9496 0 Td
(MB.)Tj
/F11 1 Tf
-10.0205 -2.126 Td
(Competing)Tj
4.3795 0 Td
(interests)Tj
3.3307 0 Td
(:)Tj
/F12 1 Tf
0.4819 0 Td
(The)Tj
1.5875 0 Td
(authors)Tj
3.0401 0 Td
(have)Tj
1.963 0 Td
(declared)Tj
-14.7827 -1.3749 Td
(that)Tj
1.6228 0 Td
(no)Tj
1.1693 0 Td
(competing)Tj
4.1599 0 Td
(interests)Tj
3.3874 0 Td
(exist.)Tj
/F11 1 Tf
-10.3394 -2.126 Td
(Abbreviati)Tj
3.9118 0 Td
(ons:)Tj
/F12 1 Tf
1.8425 0 Td
(SNP,)Tj
2.0835 0 Td
(Single-Nucle)Tj
4.6843 0 Td
(otide)Tj
-12.5221 -1.3748 Td
(Polymorphi)Tj
4.2874 0 Td
(sm;)Tj
1.6228 0 Td
(RAD,)Tj
2.1402 0 Td
(Restriction)Tj
4.2237 0 Td
(site)Tj
1.5094 0 Td
(Associated)Tj
-13.7835 -1.3748 Td
(DNA;)Tj
2.1472 0 Td
(PCA,)Tj
2.0197 0 Td
(principal)Tj
3.3662 0 Td
(component)Tj
4.4433 0 Td
(analysis)Tj
2.9623 0 Td
(;)Tj
0.4464 0 Td
(KASP,)Tj
-15.3851 -1.3748 Td
(Kompetitive)Tj
4.6063 0 Td
(Allele)Tj
2.1685 0 Td
(Specific)Tj
ET
endstream
endobj
46 0 obj
<>stream
endstream
endobj
47 0 obj
<>stream
endstream
endobj
48 0 obj
<>stream
endstream
endobj
49 0 obj
<>stream
endstream
endobj
50 0 obj
<>stream
endstream
endobj
51 0 obj
<>stream
endstream
endobj
52 0 obj
<>stream
endstream
endobj
53 0 obj
<>stream
endstream
endobj
54 0 obj
<>stream
endstream
endobj
55 0 obj
<>stream
endstream
endobj
56 0 obj
<>stream
endstream
endobj
57 0 obj
<>stream
endstream
endobj
58 0 obj
<>stream
endstream
endobj
59 0 obj
<>stream
endstream
endobj
60 0 obj
<>stream
endstream
endobj
61 0 obj
<>stream
endstream
endobj
62 0 obj
<>stream
endstream
endobj
63 0 obj
<>stream
endstream
endobj
64 0 obj
<>stream
endstream
endobj
65 0 obj
<>stream
endstream
endobj
66 0 obj
<>stream
endstream
endobj
67 0 obj
<>stream
endstream
endobj
68 0 obj
<>stream
endstream
endobj
69 0 obj
<>stream
endstream
endobj
70 0 obj
<>stream
endstream
endobj
71 0 obj
<>stream
BT
9.9 72.9643 Td
(PCR.)Tj
ET
Q
endstream
endobj
72 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 14 0 R/Annots 77 0 R/Contents 78 0 R/TrimBox[0 0 612 792]>>
endobj
73 0 obj
<>
endobj
79 0 obj
<>stream
/CIDInit/ProcSet findresource begin
12 dict begin begincmap
/CMapName/Adobe-Identity-UCS def
/CIDSystemInfo<>def
/CMapType 2 def
1 begincodespacerange
<0000>
endcodespacerange
4 beginbfchar
<0024><0041>
<0026><0043>
<002A><0047>
<0037><0054>
endbfchar
endcmap
CMapName currentdict /CMap defineresource pop end end
endstream
endobj
80 0 obj
<>/FontDescriptor 81 0 R/DW 600>>
endobj
81 0 obj
<>
endobj
82 0 obj
<>stream
cvt a &@ fpgm62 h glyf&e5