Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/8807
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dc.contributor.authorBreen, Leighen_UK
dc.contributor.authorPhilp, Andrewen_UK
dc.contributor.authorWitard, Oliveren_UK
dc.contributor.authorJackman, Sarah Ren_UK
dc.contributor.authorSelby, Annaen_UK
dc.contributor.authorSmith, Kennethen_UK
dc.contributor.authorBaar, Keithen_UK
dc.contributor.authorTipton, Kevinen_UK
dc.date.accessioned2016-08-26T00:19:18Z-
dc.date.available2016-08-26T00:19:18Zen_UK
dc.date.issued2011-08-15en_UK
dc.identifier.urihttp://hdl.handle.net/1893/8807-
dc.description.abstractThe aim of the present study was to determine mitochondrial and myofibrillar muscle protein synthesis (MPS) when carbohydrate (CHO) or carbohydrate plus protein (C+P) beverages were ingested following prolonged cycling exercise. The intracellular mechanisms thought to regulate MPS were also investigated. In a single-blind, cross-over study, 10 trained cyclists (age 29±6 years, ˙VO2max 66.5±5.1 ml kg-1 min-1) completed two trials in a randomized order. Subjects cycled for 90 min at 77±1% ˙V O2max before ingesting aCHO(25 g of carbohydrate) or C+P (25 g carbohydrate+10 g whey protein) beverage immediately and 30 min post-exercise. A primed constant infusion of L-[ring-13C6]phenylalanine began 1.5 h prior to exercise and continued until 4 h post-exercise. Muscle biopsy samples were obtained to determine myofibrillar and mitochondrial MPS and the phosphorylation of intracellular signalling proteins. Arterialized blood samples were obtained throughout the protocol. Plasma amino acid and urea concentrations increased following ingestion ofC+P only. Seruminsulin concentration increased more for C+P than CHO. Myofibrillar MPS was ∼35% greater for C+P compared with CHO (0.087±0.007 and 0.057±0.006% h-1, respectively; P =0.025).MitochondrialMPS rates were similar for C+P and CHO (0.082±0.011 and 0.086±0.018% h-1, respectively). mTORSer2448 phosphorylation was greater for C+P compared with CHO at 4 h post-exercise (P less than 0.05). p70S6KThr389 phosphorylation increased at 4 h post-exercise for C+P (P less than 0.05), whilst eEF2Thr56 phosphorylation increased by ∼40% at 4 h post-exercise for CHO only (P less than 0.01). The present study demonstrates that the ingestion of protein in addition to carbohydrate stimulates an increase in myofibrillar, but not mitochondrial, MPS following prolonged cycling. These data indicate that the increase in myofibrillar MPS for C+P could, potentially, be mediated through p70S6K, downstream of mTOR, which in turn may suppress the rise in eEF2 on translation elongation.en_UK
dc.language.isoenen_UK
dc.publisherThe Physiological Societyen_UK
dc.relationBreen L, Philp A, Witard O, Jackman SR, Selby A, Smith K, Baar K & Tipton K (2011) The influence of carbohydrate-protein co-ingestion following endurance exercise on myofibrillar and mitochondrial protein synthesis. Journal of Physiology, 589 (16), pp. 4011-4025. https://doi.org/10.1113/jphysiol.2011.211888en_UK
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.rights.urihttp://www.rioxx.net/licenses/under-embargo-all-rights-reserveden_UK
dc.titleThe influence of carbohydrate-protein co-ingestion following endurance exercise on myofibrillar and mitochondrial protein synthesisen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2999-12-12en_UK
dc.rights.embargoreason[4011.full.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.identifier.doi10.1113/jphysiol.2011.211888en_UK
dc.citation.jtitleJournal of Physiologyen_UK
dc.citation.issn1469-7793en_UK
dc.citation.issn0022-3751en_UK
dc.citation.volume589en_UK
dc.citation.issue16en_UK
dc.citation.spage4011en_UK
dc.citation.epage4025en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emailk.d.tipton@stir.ac.uken_UK
dc.citation.date11/07/2011en_UK
dc.contributor.affiliationUniversity of Birminghamen_UK
dc.contributor.affiliationUniversity of Californiaen_UK
dc.contributor.affiliationSporten_UK
dc.contributor.affiliationUniversity of Birminghamen_UK
dc.contributor.affiliationUniversity of Nottinghamen_UK
dc.contributor.affiliationUniversity of Nottinghamen_UK
dc.contributor.affiliationUniversity of California, Davisen_UK
dc.contributor.affiliationSporten_UK
dc.identifier.isiWOS:000293909600012en_UK
dc.identifier.scopusid2-s2.0-80051642419en_UK
dc.identifier.wtid778904en_UK
dc.contributor.orcid0000-0002-5875-8397en_UK
dc.contributor.orcid0000-0002-6545-8122en_UK
dc.date.accepted2011-06-25en_UK
dcterms.dateAccepted2011-06-25en_UK
dc.date.filedepositdate2012-09-03en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorBreen, Leigh|en_UK
local.rioxx.authorPhilp, Andrew|en_UK
local.rioxx.authorWitard, Oliver|0000-0002-5875-8397en_UK
local.rioxx.authorJackman, Sarah R|en_UK
local.rioxx.authorSelby, Anna|en_UK
local.rioxx.authorSmith, Kenneth|en_UK
local.rioxx.authorBaar, Keith|en_UK
local.rioxx.authorTipton, Kevin|0000-0002-6545-8122en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2999-12-12en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||en_UK
local.rioxx.filename4011.full.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0022-3751en_UK
Appears in Collections:Faculty of Health Sciences and Sport Journal Articles

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