Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/34381
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dc.contributor.advisorMoran, Colin N-
dc.contributor.advisorIain, Gallagher J-
dc.contributor.authorMacGregor, Kirstin A-
dc.date.accessioned2022-05-30T10:53:28Z-
dc.date.issued2021-11-
dc.identifier.citationMacGregor, K. A., Gallagher, I. J., & Moran, C. N. (2021). Relationship Between Insulin Sensitivity and Menstrual Cycle Is Modified by BMI, Fitness, and Physical Activity in NHANES. The Journal of Clinical Endocrinology & Metabolism, 106(10), 2979–2990. https://doi.org/10.1210/clinem/dgab415en_GB
dc.identifier.citationMacGregor, K. A., Rodriguez-Sanchez, N., Barwell, N. D., Gallagher, I. J., Moran, C. N., & Di Virgilio, T. G. (2021). Human Subcutaneous Adipose Tissue Sampling using a Mini-liposuction Technique. Journal of Visualized Experiments, 175, 62635. https://doi.org/10.3791/62635en_GB
dc.identifier.citationMacGregor K.A., Rodriguez-Sanchez N., Di Virgilio T.G., Barwell N.D., Gallagher I.J. & Moran C.N. (2022) Changes in adipose tissue microRNA expression across the menstrual cycle in regularly menstruating females: a pilot study. Physiological Genomics, 54 (1), pp. 1-10. https://doi.org/10.1152/physiolgenomics.00088.2021en_GB
dc.identifier.urihttp://hdl.handle.net/1893/34381-
dc.description.abstractThe menstrual cycle is a fundamental biological rhythm governing physiology in females of a reproductive age. Regulated across an approximately 4-weekly duration, the menstrual cycle is characterised by cyclical fluctuations in ovarian hormones (estradiol, progesterone and testosterone) and pituitary hormones (luteinizing hormone sand follicle stimulating hormone). Ovarian hormones are metabolically active and exert key regulatory roles in metabolic control. Correspondingly, a variety of metabolic parameters undergo cyclical rhythmicity across the menstrual cycle, in association with the ovarian hormone milieu. However, female physiology is under-researched. Our understanding of variation in metabolic control across the menstrual cycle and the associated molecular mechanisms remains limited. Gaining a full understanding of how metabolic control varies across the menstrual cycle is crucial for the diagnoses, treatment and prevention of metabolic disease in females. Thus, the overall aim of this thesis is to examine cyclical variation in insulin resistance and associated metabolites across the menstrual cycle. Additionally, to examine the role of inflammatory markers and miRNAs as potential molecular mechanisms underpinning variation in metabolic control across the menstrual cycle. Chapter 2 of this thesis demonstrates that rhythmic variation in insulin sensitivity, insulin, glucose and triglyceride are mediated by body mass index, physical activity and cardiorespiratory fitness. Chapter 3 extended on these findings to identify indices of body composition, fitness and physical activity levels are key modifiable risk factors mediating the variation in glucose, triglyceride, insulin sensitivity and cholesterol profiles across the menstrual cycle. Additionally, inflammatory markers varied across the menstrual cycle and associate with metabolite concentration, thereby identifying a potential mechanism which may underpin variation in metabolic control across the menstrual cycle. To gain further insight into the molecular mechanisms underpinning observed rhythmicity in metabolic control across the menstrual cycle, Chapter 4 examines the effect of the menstrual cycle on adipose tissue microRNA expression. This determined that miR-495-5p was differentially expressed across menstrual cycle phases and miR-30c-5p was negatively associated with testosterone. Adipose tissue miRNAs with the strongest tendency for differential expression between menstrual cycle phases shared common targets related to insulin signalling pathways. Overall, this thesis contributes novel data characterising variation in metabolic control across the menstrual cycle. Finally, it identifies inflammation and miRNA expression as potential molecular mechanisms driving observed variation in metabolic control.en_GB
dc.language.isoenen_GB
dc.publisherUniversity of Stirlingen_GB
dc.rightsChapter 2 was published Open Access under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence: MacGregor, K. A., Gallagher, I. J., & Moran, C. N. (2021). Relationship Between Insulin Sensitivity and Menstrual Cycle Is Modified by BMI, Fitness, and Physical Activity in NHANES. The Journal of Clinical Endocrinology & Metabolism, 106(10), 2979–2990. https://doi.org/10.1210/clinem/dgab415 Chapter 4 is the Author's Accepted manuscript and the publisher requires a 12 month embargo which has been applied to the thesis: MacGregor K.A., Rodriguez-Sanchez N., Di Virgilio T.G., Barwell N.D., Gallagher I.J. & Moran C.N. (2022) Changes in adipose tissue microRNA expression across the menstrual cycle in regularly menstruating females: a pilot study. Physiological Genomics, 54 (1), pp. 1-10. https://doi.org/10.1152/physiolgenomics.00088.2021 The following article was reproduced as an appendix and requires a 2 year embargo which has been placed on the thesis: MacGregor, K. A., Rodriguez-Sanchez, N., Barwell, N. D., Gallagher, I. J., Moran, C. N., & Di Virgilio, T. G. (2021). Human Subcutaneous Adipose Tissue Sampling using a Mini-liposuction Technique. Journal of Visualized Experiments, 175, 62635. https://doi.org/10.3791/62635 This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_GB
dc.subjectmenstrual cycleen_GB
dc.subjectinsulin sensitivityen_GB
dc.subjectmicroRNAen_GB
dc.subjectovarian hormonesen_GB
dc.subjectmetabolismen_GB
dc.subject.lcshMenstrual cycleen_GB
dc.subject.lcshMenstruation.en_GB
dc.subject.lcshMicroRNAen_GB
dc.subject.lcshInsulin resistanceen_GB
dc.subject.lcshMetabolismen_GB
dc.subject.lcshHormonesen_GB
dc.titleExamining the relationship between menstrual cycle phase with metabolic control and adipose tissue microRNA expressionen_GB
dc.typeThesis or Dissertationen_GB
dc.type.qualificationlevelDoctoralen_GB
dc.type.qualificationnameDoctor of Philosophyen_GB
dc.rights.embargodate2023-09-28-
dc.rights.embargoreasonThis thesis includes content that requires a 24 month embargo from publication.en_GB
dc.author.emailkirstinmac4@gmail.comen_GB
dc.rights.embargoterms2023-09-28en_GB
dc.rights.embargoliftdate2023-09-28-
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