Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/33664
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dc.contributor.authorMacGregor, Kirstin Aen_UK
dc.contributor.authorRodriguez-Sanchez, Nidiaen_UK
dc.contributor.authorDi Virgilio, Thomas Gen_UK
dc.contributor.authorBarwell, Nicholas Den_UK
dc.contributor.authorGallagher, Iain Jen_UK
dc.contributor.authorMoran, Colin Nen_UK
dc.date.accessioned2021-11-26T01:28:19Z-
dc.date.available2021-11-26T01:28:19Z-
dc.date.issued2022-01en_UK
dc.identifier.urihttp://hdl.handle.net/1893/33664-
dc.description.abstractCyclical changes in hormone profiles across the menstrual cycle are associated with alterations in metabolic control. MicroRNAs (miRNA) contribute to regulating metabolic control, including adipose tissue metabolism. How fluctuations in hormonal profiles across the menstrual cycle affect adipose tissue miRNA expression remain unknown. Eleven healthy, regularly menstruating females underwent four sampling visits across their menstrual cycle. Subcutaneous abdominal adipose tissue and venous blood samples were collected each at sampling visit. Luteinizing hormone (LH) tests, calendar counting, and serum hormone concentrations were used to determine menstrual cycle phases: early-follicular (EF); late-follicular (LF); post-ovulatory (PO) and mid-luteal (ML). Serum follicle-stimulating hormone, LH, estrogen, progesterone and testosterone were determined using multiplex magnetic bead panels and enzyme-linked immunosorbent assays. Global adipose tissue miRNA expression levels were determined via microarray in a subset of participants (N=8) and 17 candidate miRNAs validated by RT-qPCR in the whole cohort (N=11). Global analysis of adipose tissue miRNA expression identified 33 miRNAs significantly altered across the menstrual cycle; however, no significant differences remained after correcting for multiple testing (p>0.05). RT-qPCR analysis of candidate miRNAs revealed miR-497-5p expression was significantly altered across the menstrual cycle (np2=0.18, p=0.03); however, post-hoc tests did not reveal any significant differences between menstrual cycle phases (p> 0.05). miR-30c-5p associated with testosterone concentration (R2=0.13, p=0.033). These pilot data indicate differences in adipose tissue miRNAs in healthy women across the menstrual cycle and a weak association with ovarian hormones. Further research in larger sample sizes is required to confirm regulation of miRNA expression across the menstrual cycle.en_UK
dc.language.isoenen_UK
dc.publisherAmerican Physiological Societyen_UK
dc.relationMacGregor KA, Rodriguez-Sanchez N, Di Virgilio TG, Barwell ND, Gallagher IJ & Moran CN (2022) Changes in adipose tissue microRNA expression across the menstrual cycle in regularly menstruating females: a pilot study. Physiological Genomics, 54 (1), pp. 1-10. https://doi.org/10.1152/physiolgenomics.00088.2021en_UK
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted for publication in Physiological Genomics published by American Physiological Society who retain copyright: https://doi.org/10.1152/physiolgenomics.00088.2021en_UK
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/en_UK
dc.subjectOvarian hormonesen_UK
dc.subjectmetabolismen_UK
dc.subjecthormoneen_UK
dc.subjectestradiolen_UK
dc.subjectprogesteroneen_UK
dc.titleChanges in adipose tissue microRNA expression across the menstrual cycle in regularly menstruating females: a pilot studyen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2022-11-20en_UK
dc.rights.embargoreason[STORRE_MC and miRNA manuscript_Final accepted version_17.10.21.pdf] Publisher requires embargo of 12 months after publication.en_UK
dc.identifier.doi10.1152/physiolgenomics.00088.2021en_UK
dc.identifier.pmid34796732en_UK
dc.citation.jtitlePhysiological Genomicsen_UK
dc.citation.issn1531-2267en_UK
dc.citation.issn1094-8341en_UK
dc.citation.volume54en_UK
dc.citation.issue1en_UK
dc.citation.spage1en_UK
dc.citation.epage10en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.author.emailkirstin.macgregor1@stir.ac.uken_UK
dc.citation.date19/11/2021en_UK
dc.contributor.affiliationSporten_UK
dc.contributor.affiliationSporten_UK
dc.contributor.affiliationSporten_UK
dc.contributor.affiliationForth Valley Royal Hospitalen_UK
dc.contributor.affiliationSporten_UK
dc.contributor.affiliationSporten_UK
dc.identifier.isiWOS:000735984100001en_UK
dc.identifier.scopusid2-s2.0-85122904156en_UK
dc.identifier.wtid1775326en_UK
dc.contributor.orcid0000-0003-3960-5704en_UK
dc.contributor.orcid0000-0002-4520-0423en_UK
dc.contributor.orcid0000-0002-8630-7235en_UK
dc.contributor.orcid0000-0001-6226-8131en_UK
dc.date.accepted2021-11-16en_UK
dcterms.dateAccepted2021-11-16en_UK
dc.date.filedepositdate2021-11-24en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorMacGregor, Kirstin A|en_UK
local.rioxx.authorRodriguez-Sanchez, Nidia|0000-0003-3960-5704en_UK
local.rioxx.authorDi Virgilio, Thomas G|0000-0002-4520-0423en_UK
local.rioxx.authorBarwell, Nicholas D|en_UK
local.rioxx.authorGallagher, Iain J|0000-0002-8630-7235en_UK
local.rioxx.authorMoran, Colin N|0000-0001-6226-8131en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2022-11-20en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||2022-11-19en_UK
local.rioxx.licencehttp://creativecommons.org/licenses/by-nc/4.0/|2022-11-20|en_UK
local.rioxx.filenameSTORRE_MC and miRNA manuscript_Final accepted version_17.10.21.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source1531-2267en_UK
Appears in Collections:Faculty of Health Sciences and Sport Journal Articles

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