Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/30836
Appears in Collections:Biological and Environmental Sciences eTheses
Title: Glycosylhydrolases and the control of mannose/galactose ratio in legume-seed galactomannan.
Author(s): Holland, David
Issue Date: 1999
Publisher: University of Stirling
Abstract: This thesis describes the work carried out and results obtained from an investigation into the relationship between the two forms of a-galactosidase found within the maturing and germinating Senna occidentalis endosperms. These two enzymes are responsible for the final mannose/galactose ratio of galactomannan within the maturing senna endosperm and the mobilisation of the galactomannan storage reserve during germination. The methods used to characterise the two a-galactosidase enzymes and the results describing the slight but significant dissimilarities are described. The methods used to isolate RNA from the maturing and germinating endosperms are shown along with a description of the RT-PCR and cloning strategies then employed. The analysis of the relationship between the cDNAs obtained from the maturing and germinating endosperms used southern blotting, 5’RACE-PCR, DNA sequencing reverse-translation and multiple sequence alignments are described. Finally the similarities between the cloned senna a-galactosidases and those obtained from the swiss-prot database are shown and the commercial uses of the agalactosidase are discussed along with the future chances of producing commercially viable transgenic galactomannan yielding crops.
Type: Thesis or Dissertation
URI: http://hdl.handle.net/1893/30836

Files in This Item:
File Description SizeFormat 
13916338.pdf10.77 MBAdobe PDFView/Open



This item is protected by original copyright



Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.