Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/30076
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dc.contributor.advisorAlbalat, Amaya-
dc.contributor.advisorMacKenzie, Simon-
dc.contributor.authorMorro-Cortès, Bernat-
dc.date.accessioned2019-09-12T12:21:19Z-
dc.date.issued2019-06-
dc.identifier.citationMorro, B., Balseiro, P., Albalat, A., Pedrosa, C., Mackenzie, S., Nakamura, S., Shimizu, M., Nilsen, T.O., Sveier, H., Ebbesson, L.O. and Handeland, S.O., 2019. Effects of different photoperiod regimes on the smoltification and seawater adaptation of seawater-farmed rainbow trout (Oncorhynchus mykiss): Insights from Na+, K+–ATPase activity and transcription of osmoregulation and growth regulation genes. Aquaculture, 507, pp.282-292.en_GB
dc.identifier.urihttp://hdl.handle.net/1893/30076-
dc.description.abstractSeawater-transferred rainbow trout (Oncorhynchus mykiss) has become an attractive aquaculture product in recent years. Industrial interest is mainly due to its resistance to infectious pancreatic necrosis and its adaptability to brackish water, which allows farming in otherwise unexploited locations. However, most practices for the aquaculture of this species have been imported from Atlantic salmon (Salmo salar) without evidence data supporting their suitability for the species. These include protocols to induce their preadaptation to seawater (smoltification). As a result, following seawater transfer, large numbers of fish die (around 10%) or become growth-stunted (GS; 10-60%). Therefore, species-specific rearing protocols and seawater-readiness biomarkers are needed. In the present PhD thesis, the effects of different photoperiod and temperature protocols for rearing in freshwater were assessed on the development of smoltification traits and subsequent seawater performance. This was achieved by using an array of molecular tools to measure osmoregulation-, growth- and haematopoiesis-related genes, proteins and hormones. Moreover, the discovery of potential seawater-readiness biomarkers and the study of smoltification, seawater adaptation and GS fish development were performed using several mass spectrometry proteomic and lipidomic approaches. Results suggest that winter light signals are inadvisable for the species, while all tested summer signals produced similar good results. Moreover, increased temperature protocols not only failed to improve smoltification and growth but potentially compromised the immune system of the fish. Overall, continuous light seems an advisable light regime, irrespective of temperature. Moreover, new promising potential biomarkers for seawater-readiness were identified using proteomics, while also suggesting a previously unknown role of these proteins in smoltification and seawater adaptation. Finally, GS development was shown to be related to low insulin-like growth factor 1 levels following seawater transfer. Moreover, other related factors to the phenotype were higher stress levels, possibly caused by bullying by bigger fish, and hepatic anomalies related to oxidative stress.en_GB
dc.language.isoenen_GB
dc.publisherUniversity of Stirlingen_GB
dc.subjectAquacultureen_GB
dc.subjectSteelhead trouten_GB
dc.subjectmass spectrometryen_GB
dc.subjectATPaseen_GB
dc.subjectMALDI-TOFen_GB
dc.subjectLC-MS/MSen_GB
dc.subjectPhotoperioden_GB
dc.subjectTemperatureen_GB
dc.subjectDesmoltificationen_GB
dc.subjectBiological clocken_GB
dc.subjectHaematopoiesisen_GB
dc.subjectProteomicsen_GB
dc.subjectGrowth-stunted fishen_GB
dc.subjectRunten_GB
dc.subjectIGFen_GB
dc.subjectIGFbpen_GB
dc.subjectSomatotropic axisen_GB
dc.subjectProductionen_GB
dc.subject.lcshRainbow trouten_GB
dc.subject.lcshFishes Geneticsen_GB
dc.subject.lcshFishes Physiologyen_GB
dc.subject.lcshFishes Effect of temperature onen_GB
dc.titleThe Physiology of Smoltification and Seawater Adaptation in Rainbow Trouten_GB
dc.typeThesis or Dissertationen_GB
dc.type.qualificationlevelDoctoralen_GB
dc.type.qualificationnameDoctor of Philosophyen_GB
dc.rights.embargodate2020-12-01-
dc.rights.embargoreasonChapter 4 and 6 still haven't been published in peer -reviewed journalsen_GB
dc.contributor.funderThis work was supported by the Regionale Foskningsfond Vestalandet project: Utvikling av en sesonguavhengig protokoll for intensiv produksjon av regnbueørret (O. mykiss) (Development of a season independent protocol for the intensive production of rainbow trout (O. mykiss)) (RFFVest project 248020), the CtrlAQUA SFI, Centre for Closed-Containment Aquaculture programme (SFI project 237856), and by Japan Society for the Promotion of Science Bilateral Joint Research Project (Open Partnership with Norway). This work also received funding from the MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland; grants SASG9 and SG506) and their support is gratefully acknowledged. MASTS is funded by the Scottish Funding Council (grant reference HR09011) and contributing institutions.en_GB
dc.author.emailbernat.morro@stir.ac.uken_GB
dc.rights.embargoterms2020-12-02en_GB
dc.rights.embargoliftdate2020-12-02-
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