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Appears in Collections:Faculty of Health Sciences and Sport Journal Articles
Peer Review Status: Refereed
Title: The acute transcriptional response to resistance exercise: Impact of age and contraction mode
Author(s): Deane, Colleen S
Ames, Ryan M
Phillips, Bethan E
Weedon, Michael N
Willis, Craig R G
Boereboom, Catherine
Abdulla, Haitham
Bukhari, Syed S I
Lund, Jonathan N
Williams, John P
Wilkinson, Daniel J
Smith, Kenneth
Gallagher, Iain J
Kadi, Fawzi
Szewczyk, Nathaniel J
Issue Date: Apr-2019
Citation: Deane CS, Ames RM, Phillips BE, Weedon MN, Willis CRG, Boereboom C, Abdulla H, Bukhari SSI, Lund JN, Williams JP, Wilkinson DJ, Smith K, Gallagher IJ, Kadi F & Szewczyk NJ (2019) The acute transcriptional response to resistance exercise: Impact of age and contraction mode. Aging, 11 (7), pp. 2111-2126.
Abstract: Optimization of resistance exercise (RE) remains a hotbed of research for muscle building and maintenance. However, the interactions between the contractile components of RE (i.e. concentric (CON) and eccentric (ECC)) and age, are poorly defined. We used transcriptomics to compare age-related molecular responses to acute CON and ECC exercise. Eight young (21±1 y) and eight older (70±1 y) exercise-naïve male volunteers had vastus lateralis biopsies collected at baseline and 5 h post unilateral CON and contralateral ECC exercise. RNA was subjected to next-generation sequencing and differentially expressed (DE) genes tested for pathway enrichment using Gene Ontology (GO). The young transcriptional response to CON and ECC was highly similar and older adults displayed moderate contraction-specific profiles, with no GO enrichment. Age-specific responses to ECC revealed 104 DE genes unique to young, and 170 DE genes in older muscle, with no GO enrichment. Following CON, 15 DE genes were young muscle-specific, whereas older muscle uniquely expressed 147 up-regulated genes enriched for cell adhesion and blood vessel development, and 28 down-regulated genes involved in mitochondrial respiration, amino acid and lipid metabolism. Thus, older age is associated with contraction-specific regulation often without clear functional relevance, perhaps reflecting a degree of stochastic age-related dysregulation.
DOI Link: 10.18632/aging.101904
Rights: Deane et al. This is an open‐access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0 -, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Notes: Additional co-authors: Philip J Atherton , Timothy Etheridge
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