Please use this identifier to cite or link to this item:
Appears in Collections:Aquaculture eTheses
Title: Genetic population structure of Penaeus monodon using allozyme and mitochondrial DNA analysis
Author(s): Sodsuk, Srirat
Issue Date: 1996
Publisher: University of Stirling
Abstract: The giant tiger prawn {Penaeus monodon) was collected from 11 different sites throughout its range (Kenya—>Indo china—»Philippines). Individuals were analyzed for allozyme (46 loci) and mtDNA restriction fragment length polymorphisms (RFLPs) using 5 restriction enzymes. The average heterozygosity calculated from the allozyme data H = 0.027 (0.020-0.032) was similars to other studies on decapod crustacean. In total 10 polymorphic (p 0.99) loci were observed several of these {AAT-1 , ALAT*, GPI, IDHP , MDH-1 , MPI and PGM") contributing to significant differences in allele and genotype frequencies between populations. No significant differences were observed between samples collected at the same site in different years and no significant differences in HWE were observed within any single population. Genetic difference between all populations F^,=0.384 was high but was much smaller for SE Asian populations alone F, =0.014. The allozyme data suggested that the P. monodon populations structure could be divided into 4 main groups: Kenya, Philippines, Andaman Sea, and South China Sea. The mtDNA genome was estimated to be 15.76±.57 kb. Only four enzymes could be reliable used (BamHI, EcoKV, PvuII and SacI) which gave 12 mtDNA haplotypes (Kenya population not included). Significant differences between haplotype frequencies were observed between the Andaman Sea and South China Sea populations. Nucleotide divergence data suggested two main clonal forms of mtDNA were present in SE Asian populations. These clones appear to have diverged gene 0.1-0.7 million years ago. Clone A was more common in Andaman Sea population whereas Clone B was more common in South China Sea. Both techniques suggested that P. monodon can be subdivided into at least 3 isolated groups: Kenya, Andaman Sea, and South China Sea populations. This pattern of isolation we see today may be a relic of past separation during the last glaciation. Which has been modified by recolonization of new areas as the sea levels rose and present day patterns of gene flow caused by prevailing current patterns.
Type: Thesis or Dissertation

Files in This Item:
File Description SizeFormat 
Sodsuk.pdf15.68 MBAdobe PDFView/Open

This item is protected by original copyright

Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

The metadata of the records in the Repository are available under the CC0 public domain dedication: No Rights Reserved

If you believe that any material held in STORRE infringes copyright, please contact providing details and we will remove the Work from public display in STORRE and investigate your claim.