Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/26904
Appears in Collections:Faculty of Health Sciences and Sport Journal Articles
Peer Review Status: Refereed
Title: Primary paediatric bronchial airway epithelial cell in vitro responses to environmental exposures
Author(s): McInnes, Neil
Davidson, Matthew
Scaife, Alison
Miller, David
Spiteri, Daniella
Engelhardt, Tom
Semple, Sean
Devereux, Graham
Turner, Steve
Keywords: children’s health
epithelial cell
environmental exposures
house dust mite
interleukin-8
lipopolysaccharide
tobacco smoke
Issue Date: Apr-2016
Citation: McInnes N, Davidson M, Scaife A, Miller D, Spiteri D, Engelhardt T, Semple S, Devereux G & Turner S (2016) Primary paediatric bronchial airway epithelial cell in vitro responses to environmental exposures, International Journal of Environmental Research and Public Health, 13 (4), Art. No.: 359.
Abstract: The bronchial airway epithelial cell (BAEC) is the site for initial encounters between inhaled environmental factors and the lower respiratory system. Our hypothesis was that release of pro inflammatory interleukins (IL)-6 and IL-8 from primary BAEC cultured from children will be increased after in vitro exposure to common environmental factors. Primary BAEC were obtained from children undergoing clinically indicated routine general anaesthetic procedures. Cells were exposed to three different concentrations of lipopolysaccharide (LPS) or house dust mite allergen (HDM) or particulates extracted from side stream cigarette smoke (SSCS). BAEC were obtained from 24 children (mean age 7.0 years) and exposed to stimuli. Compared with the negative control, there was an increase in IL-6 and IL-8 release after exposure to HDM (p ≤ 0.001 for both comparisons). There was reduced IL-6 after higher compared to lower SSCS exposure (p = 0.023). There was no change in BAEC release of IL-6 or IL-8 after LPS exposure. BAEC from children are able to recognise and respond in vitro with enhanced pro inflammatory mediator secretion to some inhaled exposures. © 2016 by the authors; licensee MDPI, Basel, Switzerland.
DOI Link: http://dx.doi.org/10.3390/ijerph13040359
Rights: © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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