Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/25550
Full metadata record
DC FieldValueLanguage
dc.contributor.authorJeromson, Stewarten_UK
dc.contributor.authorMackenzie, Ivoren_UK
dc.contributor.authorDoherty, Mary Ken_UK
dc.contributor.authorWhitfield, Phillip Den_UK
dc.contributor.authorBell, J Gordonen_UK
dc.contributor.authorDick, James Ren_UK
dc.contributor.authorShaw, Andyen_UK
dc.contributor.authorRao, Francescoen_UK
dc.contributor.authorAshcroft, Stephen Pen_UK
dc.contributor.authorPhilp, Andrewen_UK
dc.contributor.authorGalloway, S Den_UK
dc.contributor.authorGallagher, Iain Jen_UK
dc.contributor.authorHamilton, David Leeen_UK
dc.date.accessioned2017-06-28T23:28:07Z-
dc.date.available2017-06-28T23:28:07Z-
dc.date.issued2018-06-30en_UK
dc.identifier.urihttp://hdl.handle.net/1893/25550-
dc.description.abstractIn striated muscle, EPA and DHA have differential effects on the metabolism of glucose and differential effects on the metabolism of protein. We have shown that, despite similar incorporation, treatment of C2C12 myotubes (CM) with EPA but not DHA improves glucose uptake and protein accretion. We hypothesized that these differential effects of EPA and DHA may be due to divergent shifts in lipidomic profiles leading to altered proteomic profiles. We therefore carried out an assessment on the impact of treating CM with EPA and DHA on lipidomic and proteomic profiles. FAME analysis revealed that both EPA and DHA led to similar but substantial changes in fatty acid profiles. Global lipidomic analysis showed that EPA and DHA induced large alterations in the cellular lipid profiles and in particular, the phospholipid classes. Subsequent targeted analysis confirmed that the most differentially regulated species were phosphatidylcholines and phosphatidylethanolamines containing long chain fatty acids with 5 (EPA treatment) or 6 (DHA treatment) double bonds. As these are typically membrane associated lipid species we hypothesized that these treatments differentially altered the membrane-associated proteome. SILAC based proteomics of the membrane fraction revealed significant divergence in the effects of EPA and DHA on the membrane associated proteome. We conclude that the EPA specific increase in polyunsaturated long chain fatty acids in the phospholipid fraction is associated with an altered membrane associated proteome and these may be critical events in the metabolic remodelling induced by EPA treatment.en_UK
dc.language.isoenen_UK
dc.publisherAmerican Physiological Societyen_UK
dc.relationJeromson S, Mackenzie I, Doherty MK, Whitfield PD, Bell JG, Dick JR, Shaw A, Rao F, Ashcroft SP, Philp A, Galloway SD, Gallagher IJ & Hamilton DL (2018) Lipid remodelling and an altered membrane-associated proteome may drive the differential effects of EPA and DHA treatment on skeletal muscle glucose uptake and protein accretion. American Journal of Physiology - Endocrinology and Metabolism, 314 (6), pp. E605-E619. https://doi.org/10.1152/ajpendo.00438.2015en_UK
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Publisher policy allows this work to be made available in this repository. Published in AJP - Endocrinology and Metabolism by American Physiological Society. The original publication is available at: https://doi.org/10.1152/ajpendo.00438.2015en_UK
dc.subjectcell signallingen_UK
dc.subjectfish oilen_UK
dc.subjectfatty aciden_UK
dc.subjectinsulinen_UK
dc.subjectlipidomicsen_UK
dc.subjectlipidsen_UK
dc.titleLipid remodelling and an altered membrane-associated proteome may drive the differential effects of EPA and DHA treatment on skeletal muscle glucose uptake and protein accretionen_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreason[ajpendo.00438.2015.full.pdf] Publisher requires embargo of 12 months after formal publication.en_UK
dc.identifier.doi10.1152/ajpendo.00438.2015en_UK
dc.identifier.pmid28655718en_UK
dc.citation.jtitleAmerican journal of physiology. Endocrinology and metabolismen_UK
dc.citation.issn1522-1555en_UK
dc.citation.issn0193-1849en_UK
dc.citation.volume314en_UK
dc.citation.issue6en_UK
dc.citation.spageE605en_UK
dc.citation.epageE619en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.contributor.funderSociety for Endocrinologyen_UK
dc.author.emaild.l.hamilton@stir.ac.uken_UK
dc.citation.date27/06/2017en_UK
dc.contributor.affiliationSporten_UK
dc.contributor.affiliationUniversity of the Highlands and Islandsen_UK
dc.contributor.affiliationUniversity of the Highlands and Islandsen_UK
dc.contributor.affiliationUniversity of the Highlands and Islandsen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
dc.contributor.affiliationDundee Cell Productsen_UK
dc.contributor.affiliationUniversity of Birminghamen_UK
dc.contributor.affiliationUniversity of Birminghamen_UK
dc.contributor.affiliationSporten_UK
dc.contributor.affiliationSporten_UK
dc.contributor.affiliationSporten_UK
dc.identifier.isiWOS:000441171500008en_UK
dc.identifier.scopusid2-s2.0-85048189010en_UK
dc.identifier.wtid525391en_UK
dc.contributor.orcid0000-0002-1622-3044en_UK
dc.contributor.orcid0000-0002-8630-7235en_UK
dc.contributor.orcid0000-0002-5620-4788en_UK
dc.date.accepted2017-06-13en_UK
dcterms.dateAccepted2017-06-13en_UK
dc.date.filedepositdate2017-06-28en_UK
dc.relation.funderprojectInvestigating the impact of Docosapentaenoic Acid (DPA) on skeletal muscle insulin sensitivityen_UK
dc.relation.funderrefn/aen_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorJeromson, Stewart|en_UK
local.rioxx.authorMackenzie, Ivor|en_UK
local.rioxx.authorDoherty, Mary K|en_UK
local.rioxx.authorWhitfield, Phillip D|en_UK
local.rioxx.authorBell, J Gordon|en_UK
local.rioxx.authorDick, James R|en_UK
local.rioxx.authorShaw, Andy|en_UK
local.rioxx.authorRao, Francesco|en_UK
local.rioxx.authorAshcroft, Stephen P|en_UK
local.rioxx.authorPhilp, Andrew|en_UK
local.rioxx.authorGalloway, S D|0000-0002-1622-3044en_UK
local.rioxx.authorGallagher, Iain J|0000-0002-8630-7235en_UK
local.rioxx.authorHamilton, David Lee|0000-0002-5620-4788en_UK
local.rioxx.projectn/a|Society for Endocrinology|http://dx.doi.org/10.13039/501100000382en_UK
local.rioxx.freetoreaddate2018-06-28en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||2018-06-27en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/all-rights-reserved|2018-06-28|en_UK
local.rioxx.filenameajpendo.00438.2015.full.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0193-1849en_UK
Appears in Collections:Faculty of Health Sciences and Sport Journal Articles

Files in This Item:
File Description SizeFormat 
ajpendo.00438.2015.full.pdfFulltext - Accepted Version25.42 MBAdobe PDFView/Open


This item is protected by original copyright



Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

The metadata of the records in the Repository are available under the CC0 public domain dedication: No Rights Reserved https://creativecommons.org/publicdomain/zero/1.0/

If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.