Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.advisorStarkey, William-
dc.contributor.authorLester, Katherine A.-
dc.description.abstractThis project investigated the development of primary cell cultures from cod and Atlantic halibut tissues (spleen, heart, optic nerve, brain, liver and tail). By using a range of culture techniques it was concluded that enzymatic disaggregation was the most efficient technique to isolate cells and develop confluent cultures. Almost seventy attempts were made in total to establish a culture. Primary cultures were produced for all organs except liver. Eagle’s Minimal Essential Medium (E-MEM) supplemented with 10% foetal bovine serum was the best all-purpose media of use. Collagen coated tissue culture flasks greatly enhanced attachment of heart cells and poly-Dlysine was beneficial to brain cell attachment. Use of the growth factors epidermal growth factor and endothelial cell growth supplement did not increase the growth of primary cultures but did increase growth in the established cell line CHSE-214 (Chinook salmon embryo). The affect of the chemical mutagen 4-nitroquinoline-N-oxide (4NQO) and two mitogens (calcium ionophore and concanavalin A) on primary cultures was inconclusive. Unusually a few of the heart cultures exhibited spontaneous contractile ability. Unfortunately although the primary cultures of tail, brain and heart were sub-cultured up to four times, they lost their proliferative potential and eventually died.en
dc.publisherUniversity of Stirlingen
dc.subjectprimary cultureen
dc.subjecttissue cultureen
dc.subject.lcshCell cultureen
dc.subject.lcshTissue cultureen
dc.subject.lcshAtlantic coden
dc.subject.lcshAtlantic halibuten
dc.titlePrimary cell cultures from cod (Gadus morhua) and Atlantic halibut (Hippoglossus hippoglossus).en
dc.typeThesis or Dissertationen
dc.type.qualificationnameMaster of Philosophyen
dc.contributor.affiliationSchool of Natural Sciences-
Appears in Collections:Aquaculture eTheses

Files in This Item:
File Description SizeFormat 
MscThesis.pdf1.51 MBAdobe PDFView/Open

This item is protected by original copyright

Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

The metadata of the records in the Repository are available under the CC0 public domain dedication: No Rights Reserved

If you believe that any material held in STORRE infringes copyright, please contact providing details and we will remove the Work from public display in STORRE and investigate your claim.