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dc.contributor.advisorWyman, Michael-
dc.contributor.authorMoffat, Christopher-
dc.description.abstractPhytoplankton growth is iron limited in at least 20% of the world’s oceans. Iron is an important nutrient required to synthesise enzymes necessary for photosynthesis, respiration, and nitrogen assimilation. Due to its low solubility in seawater, iron limitation of phytoplankton production has been the focus of much recent research. These organisms secrete ligands in order to solubilise the available iron, but not all of the iron dissolved in seawater is biologically available. In this study a molecular based approach was employed to investigate the acclimation of the marine haptophyte Coccolithus pelagicus to iron limitation. Using two dimensional electrophoresis, subtractive cDNA hybridisation, and RT real time PCR, changes in the proteome and in gene expression were examined. Iron limited cells were characterised by slower specific growth rates, lower chlorophyll a concentrations per unit biomass and less extensive calcification relative to iron replete cells. Addition of iron to iron limited cultures resulted in increased specific growth rates and increased chlorophyll a concentration per unit biomass. A subtracted cDNA library revealed seventeen identifiable sequences of which photosystem I protein E (PsaE), a fucoxanthin binding protein transcript, two chlorophyll binding proteins and a predicted membrane protein were shown to be up-regulated in iron-limited cells to varying extents. Two dimensional SDS PAGE revealed 11 differentially expressed proteins in iron limited cells and 1 highly expressed protein exclusive to iron replete cells. The potential utility of each of these as biomarkers of iron-limitation/iron sufficiency for natural populations of coccolithophorids like Coccolithus pelagicus is discussed.en
dc.publisherUniversity of Stirlingen
dc.subjectCoccolithus pelagicusen
dc.subjectiron limitationen
dc.subject.lcshMarine biologyen
dc.subject.lcshMarine Algaeen
dc.subject.lcshPhytoplankton Growth Measurementen
dc.subject.lcshSeawater Iron contenten
dc.titleAcclimation to Iron Limitation in the Haptophyte Coccolithus pelagicus: A Molecular Investigationen
dc.typeThesis or Dissertationen
dc.type.qualificationnameDoctor of Philosophyen
dc.rights.embargoreasonI require time to write articles for publication from my thesis and for my supervisor to pursue any further research stemming from the findings in the thesis. I request that public access to both electronic and paper copies of my thesis be delayed for the requested time period.en
dc.contributor.funderNatural Environment Research Council (NERC)en
dc.contributor.affiliationSchool of Natural Sciences-
dc.contributor.affiliationBiological and Environmental Sciences-
Appears in Collections:Biological and Environmental Sciences eTheses

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