Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorWhite, Amanda Ten_UK
dc.contributor.authorMcCurdy, Carrie Een_UK
dc.contributor.authorPhilp, Andrewen_UK
dc.contributor.authorHamilton, David Leeen_UK
dc.contributor.authorJohnson, Casey Den_UK
dc.contributor.authorSchenk, Simonen_UK
dc.description.abstractAims/hypothesis: The NAD+-dependent protein deacetylase sirtuin (SIRT)1 is thought to be a key regulator of skeletal muscle metabolism. However, its precise role in the regulation of insulin sensitivity is unclear. Accordingly, we sought to determine the effect of skeletal muscle-specific overexpression of SIRT1 on skeletal muscle insulin sensitivity and whole-body energy metabolism. Methods: At 10 weeks of age, mice with muscle-specific overexpression of SIRT1 and their wild-type littermates were fed a standard diet with free access to chow or an energy-restricted (60% of standard) diet for 20 days. Energy expenditure and body composition were measured by indirect calorimetry and magnetic resonance imaging, respectively. Skeletal muscle insulin-stimulated glucose uptake was measured ex vivo in soleus and extensor digitorum longus muscles using a 2-deoxyglucose uptake technique with a physiological insulin concentration of 360 pmol/l (60 μU/ml). Results: Sirt1 mRNA and SIRT1 protein levels were increased by approximately 100- and 150-fold, respectively, in skeletal muscle of mice with SIRT1 overexpression compared with wild-type mice. Despite this large-scale overexpression of SIRT1, body composition, whole-body energy expenditure, substrate oxidation and voluntary activity were comparable between genotypes. Similarly, skeletal muscle basal and insulin-stimulated glucose uptake were unaltered with SIRT1 overexpression. Finally, while 20 days of energy restriction enhanced insulin-stimulated glucose uptake in skeletal muscles of wild-type mice, no additional effect of SIRT1 overexpression was observed. Conclusions/interpretation: These results demonstrate that upregulation of SIRT1 activity in skeletal muscle does not affect whole-body energy expenditure or enhance skeletal muscle insulin sensitivity in young mice on a standard diet with free access to chow or in young mice on energy-restricted diets.en_UK
dc.relationWhite AT, McCurdy CE, Philp A, Hamilton DL, Johnson CD & Schenk S (2013) Skeletal muscle-specific overexpression of SIRT1 does not enhance whole-body energy expenditure or insulin sensitivity in young mice. Diabetologia, 56 (7), pp. 1629-1637.
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.subject2-Deoxyglucose uptakeen_UK
dc.subjectEnergy restrictionen_UK
dc.subjectInsulin sensitivityen_UK
dc.subjectSkeletal muscleen_UK
dc.titleSkeletal muscle-specific overexpression of SIRT1 does not enhance whole-body energy expenditure or insulin sensitivity in young miceen_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreason[Skeletal muscle-specific overexpression of SIRT1.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.affiliationUniversity of California, San Diegoen_UK
dc.contributor.affiliationUniversity of Coloradoen_UK
dc.contributor.affiliationUniversity of Birminghamen_UK
dc.contributor.affiliationUniversity of California, San Diegoen_UK
dc.contributor.affiliationUniversity of California, San Diegoen_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorWhite, Amanda T|en_UK
local.rioxx.authorMcCurdy, Carrie E|en_UK
local.rioxx.authorPhilp, Andrew|en_UK
local.rioxx.authorHamilton, David Lee|0000-0002-5620-4788en_UK
local.rioxx.authorJohnson, Casey D|en_UK
local.rioxx.authorSchenk, Simon|en_UK
local.rioxx.projectInternal Project|University of Stirling|
local.rioxx.filenameSkeletal muscle-specific overexpression of SIRT1.pdfen_UK
Appears in Collections:Faculty of Health Sciences and Sport Journal Articles

Files in This Item:
File Description SizeFormat 
Skeletal muscle-specific overexpression of SIRT1.pdfFulltext - Published Version328.4 kBAdobe PDFUnder Embargo until 3000-01-01    Request a copy

This item is protected by original copyright

Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

The metadata of the records in the Repository are available under the CC0 public domain dedication: No Rights Reserved

If you believe that any material held in STORRE infringes copyright, please contact providing details and we will remove the Work from public display in STORRE and investigate your claim.