|Appears in Collections:||Aquaculture eTheses|
|Title:||Aspects of the Atlantic salmon immune response during infection with the salmon louse, Lepeophtheirus salmonis (Krøyer, 1837)|
Bricknell, Ian R.
|Publisher:||University of Stirling|
|Abstract:||Atlantic salmon (Salmo salar) were experimentally infected with Lepeophtheirus salmonis copepodids and aspects of the host’s immune response investigated. Copepodid secretory/excretory product (SEP) produced during early settlement was analysed using fast-protein liquid chromatography (FPLC), sodium dodecyl sulphate (SDS)- electrophoresis and zymography. Following establishment and the appearance of the chalimus stages, the expression of the chemokine interleukin-8 (IL-8) in the heart, spleen, head kidney, fins, liver and pyloric cæca was investigated using real-time (quantitative) PCR (qPCR). Furthermore, the secretions of L. salmonis chalimus were analysed for the presence of the prostanoid PGE2 using commercially available enzyme-linked immunoassay (EIA) kits. Analysis of copepodid secretory/excretory product suggested that any immunosuppressive component is not proteinaceous in nature. Whilst there was a definite increase in protein concentration of SEP relative to control SEP, further analysis using subtractive chromatographic analysis did not reveal any unique fraction present in either SEP or CSEP that was absent in the other. Interleukin-8 expression levels in tissues changed following L. salmonis infection, with heart and spleen showing significant increases in IL-8 gene expression, whilst the head kidney, fins, liver and pyloric cæca showed no significant increase. The increase in splenic IL-8 expression may be linked to its role as one of the major secondary lymphoid organs. However, this is the first record of increase in IL-8 expression in cardiac tissue. The secretions of L. salmonis chalimus were found to contain quantifiable levels of PGE2, albeit in highly variable quantities. This concurs with already published findings for adult L. salmonis (see Fast, et al. 2004). It is proposed that the chalimus states us the PGE2 to modulate the hosts’ immune response at the site of attachment and feeding.|
|Type:||Thesis or Dissertation|
|Affiliation:||School of Natural Sciences|
|MPhil Thesis Complete with corrections 090603.pdf||2.87 MB||Adobe PDF||View/Open|
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