Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/13013
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dc.contributor.authorShepherd, Ashley-
dc.contributor.authorWilliams, Craig L-
dc.contributor.authorDoherty, Conor P-
dc.contributor.authorHossack, Margaret-
dc.contributor.authorPreston, Tom-
dc.contributor.authorMccoll, Kenneth E L-
dc.contributor.authorWeaver, Lawrence T-
dc.date.accessioned2014-09-14T01:40:05Z-
dc.date.issued2000-09-
dc.identifier.urihttp://hdl.handle.net/1893/13013-
dc.description.abstractBACKGROUND: Current diagnostic tests for Helicobacter pylori are invasive (endoscopy) or indirect (urea breath test, serology). AIMS: To evaluate a new enzyme immunoassay (EIA) which detects H pyloriantigens in faeces, by comparing its sensitivity and specificity in children with the 13C urea breath test (UBT). METHODS: A total of 119 children underwent a UBT and provided a faecal sample for antigen testing within seven days. After an overnight fast each child provided a pretest breath sample, and samples at 30 and 40 minutes after ingestion of 100 mg 13C labelled urea. 13C enrichment of breath was measured by isotope ratio mass spectrometry. Faeces were stored at -70°C until antigen testing, using the EIA. Samples were read spectrophotometrically at 450 nm and results were interpreted using recommended cut offs of optical density less than 0.14 as negative, ⩾0.16 as positive, with ⩾0.14 and less than 0.16 representing equivocal results. Sensitivity and specificity were calculated using the manufacturer's cut off compared with UBT. RESULTS: Sensitivity and specificity were 88% and 82%, respectively. Negative and positive predictive values were 97% and 58%. CONCLUSIONS: The EIA is an alternative, non-invasive, and easy to use method for the detection ofH pylori in children. Its high negative predictive value suggests a role in screening out uninfected children.en_UK
dc.language.isoen-
dc.publisherBMJ Publishing Group for the Royal College of Paediatrics and Child Health and the European Academy of Paediatrics-
dc.relationShepherd A, Williams CL, Doherty CP, Hossack M, Preston T, Mccoll KEL & Weaver LT (2000) Comparison of an enzyme immunoassay for the detection of Helicobacter pylori antigens in the faeces with the urea breath test, Archives of Disease in Childhood, 83 (3), pp. 268-270.-
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.-
dc.subjectHelicobacter pylorien_UK
dc.subjectfaecesen_UK
dc.subjectELISAen_UK
dc.subjecturea breath testen_UK
dc.subject.lcshHelicobacter pylori-
dc.titleComparison of an enzyme immunoassay for the detection of Helicobacter pylori antigens in the faeces with the urea breath testen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2999-12-31T00:00:00Z-
dc.rights.embargoreasonThe publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.-
dc.identifier.doihttp://dx.doi.org/10.1136/adc.83.3.268-
dc.citation.jtitleArchives of Disease in Childhood-
dc.citation.issn0003-9888-
dc.citation.volume83-
dc.citation.issue3-
dc.citation.spage268-
dc.citation.epage270-
dc.citation.publicationstatusPublished-
dc.citation.peerreviewedRefereed-
dc.type.statusPublisher version (final published refereed version)-
dc.author.emailashley.shepherd@stir.ac.uk-
dc.contributor.affiliationHS Research - Stirling-
dc.contributor.affiliationUniversity of Glasgow-
dc.contributor.affiliationUniversity of Glasgow-
dc.contributor.affiliationUniversity of Glasgow-
dc.contributor.affiliationScottish Universities Environmental Research Centre-
dc.contributor.affiliationUniversity of Glasgow-
dc.contributor.affiliationUniversity of Glasgow-
dc.rights.embargoterms2999-12-31-
dc.rights.embargoliftdate2999-12-31-
dc.identifier.isi000088995400024-
Appears in Collections:Faculty of Health Sciences and Sport Journal Articles

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