Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorShepherd, Ashleyen_UK
dc.contributor.authorWilliams, Craig Len_UK
dc.contributor.authorDoherty, Conor Pen_UK
dc.contributor.authorHossack, Margareten_UK
dc.contributor.authorPreston, Tomen_UK
dc.contributor.authorMccoll, Kenneth E Len_UK
dc.contributor.authorWeaver, Lawrence Ten_UK
dc.description.abstractBACKGROUND: Current diagnostic tests for Helicobacter pylori are invasive (endoscopy) or indirect (urea breath test, serology).AIMS: To evaluate a new enzyme immunoassay (EIA) which detects H pyloriantigens in faeces, by comparing its sensitivity and specificity in children with the 13C urea breath test (UBT).METHODS: A total of 119 children underwent a UBT and provided a faecal sample for antigen testing within seven days. After an overnight fast each child provided a pretest breath sample, and samples at 30 and 40 minutes after ingestion of 100 mg 13C labelled urea. 13C enrichment of breath was measured by isotope ratio mass spectrometry. Faeces were stored at -70°C until antigen testing, using the EIA. Samples were read spectrophotometrically at 450 nm and results were interpreted using recommended cut offs of optical density less than 0.14 as negative, ⩾0.16 as positive, with ⩾0.14 and less than 0.16 representing equivocal results. Sensitivity and specificity were calculated using the manufacturer's cut off compared with UBT.RESULTS: Sensitivity and specificity were 88% and 82%, respectively. Negative and positive predictive values were 97% and 58%.CONCLUSIONS: The EIA is an alternative, non-invasive, and easy to use method for the detection ofH pylori in children. Its high negative predictive value suggests a role in screening out uninfected children.en_UK
dc.publisherBMJ Publishing Group for the Royal College of Paediatrics and Child Health and the European Academy of Paediatricsen_UK
dc.relationShepherd A, Williams CL, Doherty CP, Hossack M, Preston T, Mccoll KEL & Weaver LT (2000) Comparison of an enzyme immunoassay for the detection of Helicobacter pylori antigens in the faeces with the urea breath test. Archives of Disease in Childhood, 83 (3), pp. 268-270.
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.subjectHelicobacter pylorien_UK
dc.subjecturea breath testen_UK
dc.subjectHelicobacter pylorien_UK
dc.titleComparison of an enzyme immunoassay for the detection of Helicobacter pylori antigens in the faeces with the urea breath testen_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreason[Shepherd_2000_Comparison_of_an_enzyme_immunoassay.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.citation.jtitleArchives of Disease in Childhooden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.affiliationHealth Sciences Research - Stirling - LEGACYen_UK
dc.contributor.affiliationUniversity of Glasgowen_UK
dc.contributor.affiliationUniversity of Glasgowen_UK
dc.contributor.affiliationUniversity of Glasgowen_UK
dc.contributor.affiliationScottish Universities Environmental Research Centreen_UK
dc.contributor.affiliationUniversity of Glasgowen_UK
dc.contributor.affiliationUniversity of Glasgowen_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorShepherd, Ashley|0000-0002-7687-4586en_UK
local.rioxx.authorWilliams, Craig L|en_UK
local.rioxx.authorDoherty, Conor P|en_UK
local.rioxx.authorHossack, Margaret|en_UK
local.rioxx.authorPreston, Tom|en_UK
local.rioxx.authorMccoll, Kenneth E L|en_UK
local.rioxx.authorWeaver, Lawrence T|en_UK
local.rioxx.projectInternal Project|University of Stirling|
Appears in Collections:Faculty of Health Sciences and Sport Journal Articles

Files in This Item:
File Description SizeFormat 
Shepherd_2000_Comparison_of_an_enzyme_immunoassay.pdfFulltext - Published Version211.18 kBAdobe PDFUnder Permanent Embargo    Request a copy

This item is protected by original copyright

Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

The metadata of the records in the Repository are available under the CC0 public domain dedication: No Rights Reserved

If you believe that any material held in STORRE infringes copyright, please contact providing details and we will remove the Work from public display in STORRE and investigate your claim.