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dc.contributor.authorMacKenzie, Matthew Gen_UK
dc.contributor.authorHamilton, David Leeen_UK
dc.contributor.authorMurray, James Ten_UK
dc.contributor.authorTaylor, Peter Men_UK
dc.contributor.authorBaar, Keithen_UK
dc.description.abstractFollowing resistance exercise in the fasted state, both protein synthesis and degradation in skeletal muscle are increased. The addition of essential amino acids potentiates the synthetic response suggesting that an amino acid sensor, which is involved in both synthesis and degradation, may be activated by resistance exercise. One such candidate protein is the class 3 phosphatidylinositol 3OH-kinase (PI3K) Vps34. To determine whether mammalian Vps34 (mVps34) is modulated by high-resistance contractions, mVps34 and S6K1 (an index of mTORC1) activity were measured in the distal hindlimb muscles of rats 0.5, 3, 6 and 18 h after acute unilateral high-resistance contractions with the contralateral muscles serving as a control. In the lengthening tibialis anterior (TA) muscle, S6K1 (0.5 h = 366.3 ± 112.08%, 3 h = 124.7 ± 15.96% and 6 h = 129.2 ± 0%) and mVps34 (3 h = 68.8 ± 15.1% and 6 h = 36.0 ± 8.79%) activity both increased, whereas in the shortening soleus and plantaris (PLN) muscles the increase was significantly lower (PLN S6K1 0.5 h = 33.1 ± 2.29% and 3 h = 47.0 ± 6.65%; mVps34 3 h = 24.5 ± 7.92%). HPLC analysis of the TA demonstrated a 25% increase in intramuscular leucine concentration in rats 1.5 h after exercise. A similar level of leucine added to C2C12 cells in vitro increased mVps34 activity 3.2-fold. These data suggest that, following high-resistance contractions, mVps34 activity is stimulated by an influx of essential amino acids such as leucine and this may prolong mTORC1 signalling and contribute to muscle hypertrophy.en_UK
dc.publisherWiley-Blackwell / Physiological Societyen_UK
dc.relationMacKenzie MG, Hamilton DL, Murray JT, Taylor PM & Baar K (2009) mVps34 is activated following high-resistance contractions. Journal of Physiology, 587 (1), pp. 253-260.
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.subjectMuscle strengthen_UK
dc.subjectIsometric exerciseen_UK
dc.titlemVps34 is activated following high-resistance contractionsen_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreason[Hamilton_2009_mVps34_is_activated.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.citation.jtitleJournal of Physiologyen_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.affiliationUniversity of Dundeeen_UK
dc.contributor.affiliationQueen's University Belfasten_UK
dc.contributor.affiliationUniversity of Dundeeen_UK
dc.contributor.affiliationUniversity of Dundeeen_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorMacKenzie, Matthew G|en_UK
local.rioxx.authorHamilton, David Lee|0000-0002-5620-4788en_UK
local.rioxx.authorMurray, James T|en_UK
local.rioxx.authorTaylor, Peter M|en_UK
local.rioxx.authorBaar, Keith|en_UK
local.rioxx.projectInternal Project|University of Stirling|
Appears in Collections:Faculty of Health Sciences and Sport Journal Articles

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