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Appears in Collections:Faculty of Health Sciences and Sport Journal Articles
Peer Review Status: Refereed
Title: Reduction in BACE1 decreases body weight, protects against diet-induced obesity and enhances insulin sensitivity in mice
Author(s): Meakin, Paul J
Harper, Alex J
Hamilton, David Lee
Gallagher, Jennifer
McNeilly, Alison D
Burgess, Laura A
Vaanholt, Lobke M
Bannon, Kirsten A
Latcham, Judy
Hussain, Ishrut
Speakman, John R
Howlett, David R
Ashford, Michael L J
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Keywords: beta-site amyloid precursor protein-cleaving enzyme 1 (BACE1)
glucose uptake
insulin sensitivity
skeletal muscle
uncoupling protein (UCP)
Evidence-Based Medicine
Diabetes Mellitus, Type 2 therapy
Issue Date: 1-Jan-2012
Date Deposited: 29-Apr-2013
Citation: Meakin PJ, Harper AJ, Hamilton DL, Gallagher J, McNeilly AD, Burgess LA, Vaanholt LM, Bannon KA, Latcham J, Hussain I, Speakman JR, Howlett DR & Ashford MLJ (2012) Reduction in BACE1 decreases body weight, protects against diet-induced obesity and enhances insulin sensitivity in mice. Biochemical Journal, 441 (1), pp. 285-296.
Abstract: Insulin resistance and impaired glucose homoeostasis are important indicators of Type 2 diabetes and are early risk factors of AD (Alzheimer's disease). An essential feature of AD pathology is the presence of BACE1 (beta-site amyloid precursor protein-cleaving enzyme 1), which regulates production of toxic amyloid peptides. However, whether BACE1 also plays a role in glucose homoeostasis is presently unknown. We have used transgenic mice to analyse the effects of loss of BACE1 on body weight, and lipid and glucose homoeostasis. BACE1-/- mice are lean, with decreased adiposity, higher energy expenditure, and improved glucose disposal and peripheral insulin sensitivity than wild-type littermates. BACE1-/- mice are also protected from diet-induced obesity. BACE1-deficient skeletal muscle and liver exhibit improved insulin sensitivity. In a skeletal muscle cell line, BACE1 inhibition increased glucose uptake and enhanced insulin sensitivity. The loss of BACE1 is associated with increased levels of UCP1 (uncoupling protein 1) in BAT (brown adipose tissue) and UCP2 and UCP3 mRNA in skeletal muscle, indicative of increased uncoupled respiration and metabolic inefficiency. Thus BACE1 levels may play a critical role in glucose and lipid homoeostasis in conditions of chronic nutrient excess. Therefore strategies that ameliorate BACE1 activity may be important novel approaches for the treatment of diabetes.
DOI Link: 10.1042/BJ20110512
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