|Appears in Collections:||Aquaculture Journal Articles|
|Peer Review Status:||Refereed|
|Title:||Production of heterozygous and homozygous clones in Nile tilapia|
|Authors:||Hussain, M Gulam|
heterozygous and homozygous clones
Nile tilapia (Oreochromis niloticus L.)
|Citation:||Hussain MG, Penman D & McAndrew B (1998) Production of heterozygous and homozygous clones in Nile tilapia, Aquaculture International, 6 (3), pp. 197-205.|
|Abstract:||Production of heterozygous and homozygous clones of Nile tilapia (Oreochromis niloticus L.) was successfully carried out. An outbred clonal line was produced by cross-breeding between a viable mitotic gynogenetic female and male (recessive mutation in a sex determining gene). At the same time, an inbred clonal line was produced by gynogenetic reproduction (retention of 2nd polar body) using optimal pressure/heat shock treatments. The heterozygous and homozygous nature of the two types of clonal lines were checked and identified at the adenosine deaminase ADA* marker locus. A model for the large scale production of similar clones is presented. The possible implications of such clonal lines in genetic research and in the culture of Oreochromis spp. are discussed.|
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