Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/9937
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Linkage relationships and haplotype polymorphism among cichlid Mhc class II B loci
Authors: Malaga-Trillo, Edward
Zaleska-Rutczynska, Zofia
McAndrew, Brendan
Vincek, Vladimir
Figueroa, Felipe
Sultmann, Holger
Klein, Jan
Contact Email: b.j.mcandrew@stir.ac.uk
Issue Date: Jul-1998
Publisher: The Genetics Society of America
Citation: Malaga-Trillo E, Zaleska-Rutczynska Z, McAndrew B, Vincek V, Figueroa F, Sultmann H & Klein J (1998) Linkage relationships and haplotype polymorphism among cichlid Mhc class II B loci, Genetics, 149 (3), pp. 1527-1537.
Abstract: The species flocks of cichlid fishes in the Great East African Lakes are paradigms of adaptive radiation and hence, of great interest to evolutionary biologists. Phylogenetic studies of these fishes have, however, been hampered by the lack of suitable polymorphic markers. The genes of the major histocompatibility complex hold the promise to provide, through their extensive polymorphism, a large number of such markers, but their use has been hampered by the complexity of the genetic system and the lack of definition of the individual loci. In this study we take the first substantial step to alleviate this problem. Using a combination of methods, including the typing of single sperm cells, gyno- or androgenetic individuals, and haploid embryos, as well as sequencing of class II B restriction fragments isolated from gels for Southern blots, we identify the previously characterized homology groups as distinct loci. At least 17 polymorphic class II B loci, all of which are presumably transcribed, have been found among the different species studied. Most of these loci are shared across the various cichlid species and genera. The number of loci per haplotype varies from individual to individual, ranging from 1 to 13. A total of 21 distinct haplotypes differing in the number of loci they carry has thus far been identified. All the polymorphic loci are part of the same cluster in which, however, distances between at least some of the loci (as indicated by recombination frequencies) are relatively large. Both the individual loci and the haplotypes can now be used to study phylogenetic relationships among the members of the species flocks and the mode in which speciation occurs during adaptive radiation.
Type: Journal Article
URI: http://hdl.handle.net/1893/9937
URL: http://www.genetics.org/content/149/3/1527.abstract
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Max Planck Institute for Biology
Max Planck Institute for Biology
Aquaculture
University of Miami, USA
Max Planck Institute for Biology
Max Planck Institute for Biology
Max Planck Institute for Biology

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