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Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/9893
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dc.contributor.authorEzaz, M Tariqen_UK
dc.contributor.authorHarvey, Simon Cen_UK
dc.contributor.authorBoonphakdee, Chutaen_UK
dc.contributor.authorTeale, Alan Jen_UK
dc.contributor.authorMcAndrew, Brendanen_UK
dc.contributor.authorPenman, Daviden_UK
dc.date.accessioned2012-11-06T23:15:55Z-
dc.date.available2012-11-06T23:15:55Zen_UK
dc.date.issued2004-09en_UK
dc.identifier.urihttp://hdl.handle.net/1893/9893-
dc.description.abstractGynogenetically produced XX and YY Nile tilapia (Oreochromis niloticus) and diploid control groups were screened for amplified fragment length polymorphisms (AFLPs) to search for sex-linked or sex-specific markers. Family-level bulked segregant analysis (XX and YY gynogenetic family pools) and individual screening (XX and YY gynogenetics and XX and XY control individuals) identified 3 Y-linked (OniY425, OniY382, OniY227) and one X-linked (OniX420) AFLP markers. OniX420 and OniY425 were shown to be allelic. Single locus polymerase chain reaction assays were developed for these markers. Tight linkage was demonstrated between the AFLP markers and the sex locus within the source families. However, these markers failed to consistently identify sex in unrelated individuals, indicating recombination between the markers and the sexdetermining loci. O. niloticus bacterial artificial chromosome clones, containing the AFLP markers, hybridized to the long arm of chromosome 1. This confirmed previous evidence, based on meiotic chromosome pairing and fluorescence in situ hybridization probes obtained through chromosome microdissection, that chromosome pair 1 is the sex chromosomes.en_UK
dc.language.isoenen_UK
dc.publisherSpringeren_UK
dc.relationEzaz MT, Harvey SC, Boonphakdee C, Teale AJ, McAndrew B & Penman D (2004) Isolation and physical mapping of sex-linked AFLP markers in nile tilapia (Oreochromis niloticus L.). Marine Biotechnology, 6 (5), pp. 435-445. https://doi.org/10.1007/s10126-004-3004-6en_UK
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.rights.urihttp://www.rioxx.net/licenses/under-embargo-all-rights-reserveden_UK
dc.subjectNile tilapiaen_UK
dc.subjectOreochromis niloticusen_UK
dc.subjectsex determinationen_UK
dc.subjectsex linkageen_UK
dc.subjectAFLP markersen_UK
dc.subjectFISH mappingen_UK
dc.subjectSustainable aquacultureen_UK
dc.subjectNile tilapiaen_UK
dc.titleIsolation and physical mapping of sex-linked AFLP markers in nile tilapia (Oreochromis niloticus L.)en_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2999-12-31en_UK
dc.rights.embargoreason[ezazetal_MB_2004.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.identifier.doi10.1007/s10126-004-3004-6en_UK
dc.citation.jtitleMarine Biotechnologyen_UK
dc.citation.issn1436-2236en_UK
dc.citation.issn1436-2228en_UK
dc.citation.volume6en_UK
dc.citation.issue5en_UK
dc.citation.spage435en_UK
dc.citation.epage445en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emailb.j.mcandrew@stir.ac.uken_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isiWOS:000226726700005en_UK
dc.identifier.scopusid2-s2.0-10844258770en_UK
dc.identifier.wtid760702en_UK
dc.contributor.orcid0000-0001-7384-5133en_UK
dc.contributor.orcid0000-0001-8608-6631en_UK
dcterms.dateAccepted2004-09-30en_UK
dc.date.filedepositdate2012-10-31en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorEzaz, M Tariq|en_UK
local.rioxx.authorHarvey, Simon C|en_UK
local.rioxx.authorBoonphakdee, Chuta|en_UK
local.rioxx.authorTeale, Alan J|en_UK
local.rioxx.authorMcAndrew, Brendan|0000-0001-7384-5133en_UK
local.rioxx.authorPenman, David|0000-0001-8608-6631en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2999-12-31en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||en_UK
local.rioxx.filenameezazetal_MB_2004.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source1436-2228en_UK
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