Detection of hybridization between Chinese carp species (Hypophthalmichthys molitrix and Aristichthys nobilis) in hatchery broodstock in Bangladesh, using DNA microsatellite loci

Abstract

Hybridization between silver carp (Hypophthalmichthys molitrix) and bighead carp (Aristichthys nobilis) has been reported to occur fairly frequently in commercial aquaculture hatcheries in Bangladesh. The consequences of hybridization for broodstock purity had not previously been investigated. Allelic variation at three microsatellite DNA loci isolated from silver carp routinely distinguished between silver carp and bighead carp. These markers were used in the analysis of samples collected from hatcheries in different regions of Bangladesh. Of 422 hatchery broodstock that were morphologically identified as silver carp, 8.3% had bighead allele(s) at one or more of the three microsatellite loci, while 23.3% of the 236 fish morphologically identified as bighead carp had silver carp allele(s) at one or more loci. The results suggested that while some of these fish might be F1 hybrids, others had more complex genotypes, suggesting further generations of hybridization or introgression between the species in hatcheries, with potentially damaging consequences for the integrity of these stocks and their performance in aquaculture.