Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/9850
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Functionally significant allelic variation in myosin light chain composition in a tropical cichlid
Authors: Crockford, Tony
Johnston, Ian A
McAndrew, Brendan
Contact Email: b.j.mcandrew@stir.ac.uk
Keywords: muscle contraction
myosin
fish
allelic variation
tilapia
Oreochromis niloticus
Oreochromis andersonii
Issue Date: Dec-1995
Publisher: The Company of Biologists Ltd
Citation: Crockford T, Johnston IA & McAndrew B (1995) Functionally significant allelic variation in myosin light chain composition in a tropical cichlid, Journal of Experimental Biology, 198 (12), pp. 2501-2508.
Abstract: Single fast muscle fibres in the tropical fish Oreochromis andersonii were found to contain two myosin light chains (LC1s; LC1f1* or LC1f2*). Breeding experiments confirmed that the different LC1s were of allelic origin and their inheritance patterns conformed to Mendelian expectations (1:2:1). The LC1s differed in apparent relative molecular mass by 800-900. No other differences in myosin subunits were found between the LC1 genotypes. The molar ratios of LC3:LC1(total) in the fast muscle of O. andersonii homozygous for LC1f1* or LC1f2* and heterozygous for both alleles were 2.0:1, 2.1:1 and 2.2:1, respectively, as determined by capillary electrophoresis. The maximum contraction velocity (Vmax) of single skinned muscle fibres was determined at 20 °C by the slack-test method. Vmax values (fibre lengths s21) for fast muscle fibres from O. andersonii which were homozygous for either LC1f2* or LC1f1* were 5.3 and 3.3, respectively, compared with 3.8 when both alleles were present. Crosses between Oreochromis niloticus and O. andersonii produced F1 hybrids which were heterozygous for either LC1n/LC1f1* or LC1n/LC1f2*, where LC1n is the myosin light chain for O. niloticus. The distribution of myosin light chain genotypes in hybrid offspring was not significantly different from the expected Mendelian 1:1 ratio (47%: 53%). The Vmax (fibre lengths s21) of muscle fibres containing LC1f2* from hybrid Oreochromis was 4.3 compared with 3.1 for the LC1f1* genotype. The results are consistent with a functionally significant allelic variation in myosin LC1 in fast muscle fibres from O. andersonii which is also expressed in hybrid genotypes.
Type: Journal Article
URI: http://hdl.handle.net/1893/9850
URL: http://jeb.biologists.org/content/198/12/2501.abstract
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: University of St Andrews
University of St Andrews
Aquaculture

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