Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/9117
Appears in Collections:Aquaculture Book Chapters and Sections
Peer Review Status: Refereed
Title: Chapter 2 – Utility of greater wax moth larva (galleria mellonella) for evaluating the toxicity and efficacy of new antimicrobial agen
Authors: Desbois, Andrew P
Coote, Peter J
Contact Email: andrew.desbois@stir.ac.uk
Editors: Laskin, AI
Sariaslani, S
Gadd, GM
Citation: Desbois AP & Coote PJ (2012) Chapter 2 – Utility of greater wax moth larva (galleria mellonella) for evaluating the toxicity and efficacy of new antimicrobial agents. In: Laskin AI, Sariaslani S, Gadd GM (ed.). Advances in Applied Microbiology, Volume 78. Advances in Applied Microbiology, 78, Amsterdam: Elsevier, pp. 25-5
Keywords: Wax moth larva
Antimicrobial agents
Virulence
Minimum inhibitory concentration
Issue Date: 2012
Publisher: Elsevier
Series/Report no.: Advances in Applied Microbiology, 78
Abstract: There is an urgent need for new antimicrobial agents to combat infections caused by drug-resistant pathogens. Once a compound is shown to be effective in vitro, it is necessary to evaluate its efficacy in an animal infection model. Typically, this is achieved using a mammalian model, but such experiments are costly, time consuming, and require full ethical consideration. Hence, cheaper and ethically more acceptable invertebrate models of infection have been introduced, including the larvae of the greater wax moth Galleria mellonella. Invertebrates have an immune system that is functionally similar to the innate immune system of mammals, and often identical virulence and pathogenicity factors are used by human pathogenic microbes to infect wax moth larvae and mammals. Moreover, the virulence of many human pathogens is comparable in wax moth larvae and mammals. Using key examples from the literature, this chapter highlights the benefits of using the wax moth larva model to provide a rapid, inexpensive, and reliable evaluation of the toxicity and efficacy of new antimicrobial agents in vivo and prior to the use of more expensive mammalian models. This simple insect model can bridge the gap between in vitro studies and mammalian experimentation by screening out compounds with a low likelihood of success, while providing greater justification for further studies in mammalian systems. Thus, broader implementation of the wax moth larva model into anti- infective drug discovery and development programs could reduce the use of mammals during preclinical assessments and the overall cost of drug development.
Rights: The publisher has not responded to our queries therefore this work cannot be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Type: Part of book or chapter of book
URI: http://hdl.handle.net/1893/9117
URL: http://www.sciencedirect.com/science/bookseries/00652164/78
Affiliation: Aquaculture
University of St Andrews

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