|Appears in Collections:||Aquaculture Journal Articles|
|Peer Review Status:||Refereed|
|Title:||Differences in CYP1A response between flounder (Platichthys flesus) and plaice (Pleuronectes platessa) after long-term exposure to harbour dredged spoil in a mesocosm study|
|Authors:||Eggens, Martin L|
Vethaak, A Dick
Horbach, G J M Jean
Boon, Jan P
|Citation:||Eggens ML, Vethaak AD, Leaver M, Horbach GJJM, Boon JP & Seinen W (1996) Differences in CYP1A response between flounder (Platichthys flesus) and plaice (Pleuronectes platessa) after long-term exposure to harbour dredged spoil in a mesocosm study, Chemosphere, 32 (7), pp. 1357-1380.|
|Abstract:||Flounder (Platichthys flesus) and plaice (Pleuronectes platessa) were exposed to three different levels of pollution involving contaminants from harbour dredged spoil in a large-scale mesocosm study lasting 30 months. Exposure of fish to PCBs and PAHs was estimated from liver concentrations of CB-153 and biliary levels of 1-OH pyrens in flounder. Both marker compounds were significantly elevated in fish from the polluted mesocosms in comparison to the reference mesocosm. Of the three parameters used to characterise CYPIA induction in flounder, only CYPIA-mRNA in female fish was significantly induced by pollution. However, significant induction of EROD activity and immunochemically measured CYPlA protein content was observed in plaice. To investigate in more detail the apparent lack of CYP1A induction, a parallel experiment was run in which flounder were injected with a single i.p. injection of benzo[a]pyrene (BaP) and sacrificed after 48 hours. Since this approach did result in significant induction of all the parameters used to characterise CYP1A induction (EROD, CYPIA, CYPIA mRNA), it appears that external factors may have prevented induction in the mesocosm experiments. Down-regulating effects of the possible presence of a compound with oestrogenic activity, or altered membrane-CYPIA or membrane-receptor interaction, could have contributed to this apparent lack of response, despite the intrinsic ability of flounder to increase CYPIA levels after exposure to a model inducer (BaP).|
|Rights:||The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.|
|Affiliation:||National Institute for Coastal and Marine Management|
National Institute for Coastal and Marine Management
Netherlands Institute for Sea Research
|1-s2.0-004565359600046X-main.pdf||929.7 kB||Adobe PDF||Under Embargo until 31/12/2999 Request a copy|
Note: If any of the files in this item are currently embargoed, you can request a copy directly from the author by clicking the padlock icon above. However, this facility is dependant on the depositor still being contactable at their original email address.
This item is protected by original copyright
Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
If you believe that any material held in STORRE infringes copyright, please contact firstname.lastname@example.org providing details and we will remove the Work from public display in STORRE and investigate your claim.