Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/7685
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Alternatives to whole animal testing: Use of cDNA probes for studies of phase I and II enzyme induction in isolated plaice hepatocytes
Authors: Hitchman, N
Leaver, Michael
George, Stephen
Contact Email: m.j.leaver@stir.ac.uk
Issue Date: 1995
Publisher: Elsevier
Citation: Hitchman N, Leaver M & George S (1995) Alternatives to whole animal testing: Use of cDNA probes for studies of phase I and II enzyme induction in isolated plaice hepatocytes, Marine Environmental Research, 39 (1-4), pp. 289-292.
Abstract: Studies of xenobiotic metabolism and the regulation of enzyme systems for their metabolism (Phase I and II enzyme systems) require large numbers of animals, intensive use of experimental aquarium systems and in some instances can pose major problems when the compounds are scarce, expensive or too toxic to be disposed of easily. Previous studies have demonstrated the usefulness of isolated primary hepatocytes of fish for metabolic and enzyme induction studies. Functionally and structurally competent hepatocytes were isolated from juvenile plaice and after overnight acclimation to culture conditions, were exposed to a variety of PAHs for 24h. The levels of CYP1A1, phenol UDPGT and GST-A mRNAs were then estimated by slot blotting and hybridisation to their cDNA probes. The method enabled rapid and easy determination of the structure/activity relationships of these compounds as inducers of these key Phase I and II enzymes, and is potentially useful for screening large numbers of compounds both as an invitro toxicity test and for mechanistic studies.
Type: Journal Article
URI: http://hdl.handle.net/1893/7685
URL: http://www.sciencedirect.com/science/article/pii/014111369400039R
DOI Link: http://dx.doi.org/10.1016/0141-1136(94)00039-R
Rights: The publisher has not responded to our queries therefore this work cannot be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: University of Stirling
Aquaculture
Aquaculture

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