Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/7633
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dc.contributor.authorTocher, Douglas Ren_UK
dc.contributor.authorFonseca-Madrigal, Jorgeen_UK
dc.contributor.authorDick, James Ren_UK
dc.contributor.authorNg, Wing–Keongen_UK
dc.contributor.authorBell, J Gordonen_UK
dc.contributor.authorCampbell, Patricken_UK
dc.date.accessioned2017-11-24T23:42:56Z-
dc.date.available2017-11-24T23:42:56Zen_UK
dc.date.issued2004-01en_UK
dc.identifier.urihttp://hdl.handle.net/1893/7633-
dc.description.abstractFood grade fisheries have reached their sustainable limits while aquaculture production has increased to meet consumer demands. However, for growth in aquaculture to continue and utilise sustainable, feeding ingredients, alternatives to fish oil (FO), the predominant lipid component of fish diets, must be developed. Therefore, there is currently considerable interest in the regulation of fatty acid metabolism in fish in order to determine strategies for the best use of plant oils in diets for commercially important cultured fish species. Plant oils are characteristically rich in C18 polyunsaturated fatty acids (PUFA) but devoid of C20 and C22 highly unsaturated fatty acids (HUFA) found in FO. The fatty acyl desaturase enzyme activities involved in the biosynthesis of HUFA from PUFA are known to be under nutritional regulation and can be increased in fish fed diets rich in plant oils. However, fatty acid desaturase activity is also known to be modulated by water temperature in fish. The present study aimed to investigate the interaction between water temperature and diet in the regulation of fatty acid metabolism in rainbow trout. Trout, acclimatized to 7, 11 or 15 °C, were fed for 4 weeks on diets in which the FO was replaced in a graded manner by palm oil. At the end of the trial, fatty acyl desaturation/elongation and β-oxidation activities were determined in isolated hepatocytes and intestinal enterocytes using [1-14C]18:3n-3 as substrate, and samples of liver were collected for analysis of lipid and fatty acid composition. The most obvious effect of temperature was that fatty acid desaturation/elongation and β-oxidation were reduced in both hepatocytes and intestinal enterocytes from fish maintained at the highest water temperature (15 °C). There were differences between the two tissues with the highest desaturation/elongation and β-oxidation activities tending to be in fish held at 11 °C in the case of hepatocytes, but 7 °C in enterocytes. Correlations between fatty acid metabolism and dietary palm oil were most clearly observed in desaturation/elongation activities in both hepatocytes and enterocytes at 11 °C. The highest β-oxidation activities were generally observed in fish fed FO alone in both hepatocytes and enterocytes with palm oil having differential effects in the two cell types.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationTocher DR, Fonseca-Madrigal J, Dick JR, Ng W, Bell JG & Campbell P (2004) Effects of water temperature and diets containing palm oil on fatty acid desaturation and oxidation in hepatocytes and intestinal enterocytes of rainbow trout (Oncorhynchus mykiss). Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 137 (1), pp. 49-63. https://doi.org/10.1016/j.cbpc.2003.10.002en_UK
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.rights.urihttp://www.rioxx.net/licenses/under-embargo-all-rights-reserveden_UK
dc.subjectdesaturationen_UK
dc.subjectenterocytesen_UK
dc.subjecthepatocytesen_UK
dc.subjectbeta-oxidationen_UK
dc.subjectpalm oilen_UK
dc.subjectpolyunsaturated fatty acidsen_UK
dc.subjectrainbow trouten_UK
dc.titleEffects of water temperature and diets containing palm oil on fatty acid desaturation and oxidation in hepatocytes and intestinal enterocytes of rainbow trout (Oncorhynchus mykiss)en_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate3000-01-01en_UK
dc.rights.embargoreason[tocher_comparbiochemandphysio137_2004.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.identifier.doi10.1016/j.cbpc.2003.10.002en_UK
dc.citation.jtitleComparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biologyen_UK
dc.citation.issn1096-4959en_UK
dc.citation.volume137en_UK
dc.citation.issue1en_UK
dc.citation.spage49en_UK
dc.citation.epage63en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emaild.r.tocher@stir.ac.uken_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Science, Malaysia (USM)en_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationBioMar U.K.en_UK
dc.identifier.isiWOS:000188058800005en_UK
dc.identifier.scopusid2-s2.0-0348048781en_UK
dc.identifier.wtid782966en_UK
dc.contributor.orcid0000-0002-8603-9410en_UK
dcterms.dateAccepted2004-01-31en_UK
dc.date.filedepositdate2012-08-24en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorTocher, Douglas R|0000-0002-8603-9410en_UK
local.rioxx.authorFonseca-Madrigal, Jorge|en_UK
local.rioxx.authorDick, James R|en_UK
local.rioxx.authorNg, Wing–Keong|en_UK
local.rioxx.authorBell, J Gordon|en_UK
local.rioxx.authorCampbell, Patrick|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate3000-01-01en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||en_UK
local.rioxx.filenametocher_comparbiochemandphysio137_2004.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source1096-4959en_UK
Appears in Collections:Aquaculture Journal Articles

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