Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/7615

Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Evidence from heterologous expression of glutathione S-transferases A and A1 of the plaice (Pleuronectes platessa) that their endogenous role is in detoxification of lipid peroxidation products
Authors: Martinez-Lara, Esther
Leaver, Michael
George, Stephen
Contact Email: m.j.leaver@stir.ac.uk
Keywords: plaice
glutathione-S-transferase
lipid oxidation
Issue Date: Sep-2002
Publisher: Elsevier
Citation: Martinez-Lara E, Leaver M & George S (2002) Evidence from heterologous expression of glutathione S-transferases A and A1 of the plaice (Pleuronectes platessa) that their endogenous role is in detoxification of lipid peroxidation products, Marine Environmental Research, 54 (3-5), pp. 263-266.
Abstract: cDNA clones for glutathione S-transferases A (GST-A) and A1 (GST-A1) from plaice (Pleuronectes platessa) were expressed as N-terminally 6XHis tagged proteins in Escherichia coli and purified to homogeneity from Ni-NTA silica. GST-A was an efficient catalyst for conjugation of unsaturated alkenals derived from peroxidation of polyunsaturated fatty acids with the highest activity observed with trans-non-2-enal (8 μmol min-1 mg-1). GST-A1 was a very efficient Se- independent glutathione peroxidase with an activity towards cumene hydroperoxide of 25 μmol min-1 mg-1 . Although the enzymes exhibited moderately high activities towards the model substrate 1-chloro-2,4-dinitrobenzene (CDNB) they exhibited little or no activity towards other common prototypical xenobiotic substrates. Together with data for ontogeny, tissue distribution and inducibility of these enzymes, we contend that a primary function of these enzymes is protection from the harmful effects of lipid peroxidation products generated naturally or exacerbated by xenobiotic exposur
Type: Journal Article
URI: http://hdl.handle.net/1893/7615
URL: http://www.sciencedirect.com/science/article/pii/S0141113602001885
DOI Link: http://dx.doi.org/10.1016/S0141-1136(02)00188-5
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: University of Caen
Aquaculture
Aquaculture

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