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dc.contributor.authorTocher, Douglas R-
dc.contributor.authorDick, James R-
dc.contributor.authorSargent, John R-
dc.description.abstractRecently we reported the development of a fish cell line, EPC-EFAD, derived from the carp (Cyprinus carpio) epithelial papilloma line, EPC, that could survive and proliferate in essential fatty acid-deficient (EFAD) medium. The EPC-EFAD cell line may be a useful model system in which to study the cellular biochemical effects of EFA deficiency and has advantages in studies of polyunsaturated fatty acid (PUFA) and eicosanoid metabolism in fish in that the complications introduced by culture in relatively n-6 PUFA-rich mammalian sera are removed. In the present study, the effects on cell proliferation rate of supplementing EPC-EFAD cells with various n-3 and n-6 PUFA were investigated to determine the possible role(s) of PUFA in cell growth and division. The selectivity of incorporation of specific PUFA into individual glycerophospholipid classes and the feasibility of reproducing in vivo fatty acid compositions in vitro were also investigated. Proliferation of the EPC-EFAD cell line was stimulated by arachidonic (20:4 n-6), eicosapentaenoic (20:5 n-3) and docosahexaenoic (22:6 n-3) fatty acids but not by 18:2 n-6 or 18:3 n-3. The differential effects of PUFA on cellular proliferation may be related to the lack of significant Δ5 desaturase activity in the cells at 22°C and may implicate a role for eicosanoids in the mechanism of stimulation of proliferation. PUFA supplementation increased the cytotoxic effects of longer term culture, an effect that was partly alleviated by inclusion of vitamin E in the culture medium. The cells could generally be supplemented with PUFA to produce cellular fatty acid compositions in vitro that were similar to in vivo compositionen_UK
dc.relationTocher DR, Dick JR & Sargent JR (1996) Stimulation of proliferation of an essential fatty acid-deficient fish cell line by C-20 and C-22 polyunsaturated fatty acids and effects on fatty acid composition, Prostaglandins, Leukotrienes and Essential Fatty Acids, 55 (5), pp. 345-356.-
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.-
dc.titleStimulation of proliferation of an essential fatty acid-deficient fish cell line by C-20 and C-22 polyunsaturated fatty acids and effects on fatty acid compositionen_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreasonThe publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.-
dc.citation.jtitleProstaglandins, Leukotrienes and Essential Fatty Acids-
dc.type.statusPublisher version (final published refereed version)-
dc.contributor.affiliationUniversity of Stirling-
Appears in Collections:Aquaculture Journal Articles

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