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Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Comparative analysis of the phenotypic characteristics of high- and low-virulent strains of Edwardsiella tarda
Authors: Wang, Yan
Zhang, Xiao-Hua
Austin, Brian
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Keywords: Edwardsiella tarda
lipid A
SDS-polyacrylamide gel electrophoresis
Issue Date: Dec-2010
Publisher: Wiley-Blackwell
Citation: Wang Y, Zhang X & Austin B (2010) Comparative analysis of the phenotypic characteristics of high- and low-virulent strains of Edwardsiella tarda, Journal of Fish Diseases, 33 (12), pp. 985-994.
Abstract: Edwardsiella tarda is a causative agent of edwardsiellosis in freshwater and marine fish. Extracellular enzymic, haemolytic, hydrophobic and serum resistance activities, haemagglutination, autoagglutination and siderophores of high- and low- virulent E. tarda strains were examined. The results revealed different haemagglutination, autoagglutination, haemolytic, hydrophobic and serum resistance activities in different strains. Analysis of extracellular proteins (ECPs) and outer membrane proteins (OMPs) demonstrated several major, low molecular weight, virulent-strain-specific proteins, which could be virulence- related. Based on the database search with MALDI-TOF MS data, the closest homologies of the three protein bands Ed1, Ed2 and Ed3 were phosphotransferase enzyme family protein, nitrite reductase [NAD(P)H], large subunit and ATP- dependent Lon protease, respectively. A comparison of pathogenicity of purified lipopolysaccharide (LPS) and lipid A from virulent and avirulent strains demonstrated that LPS was one of the virulence factors of the E. tarda isolates, and lipid A was a biologically active determinant of LPS.
Type: Journal Article
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Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Ocean University of China
Ocean University of China
Heriot-Watt University

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