Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/7374
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Kocuria SM1 controls vibriosis in rainbow trout (Oncorhynchus mykiss, Walbaum)
Authors: Sharifuzzaman, S M
Austin, Brian
Contact Email: brian.austin@stir.ac.uk
Keywords: innate immunity
Kocuria SM1
probiotics
rainbow trout
Vibrio anguillarum
V
ordalii
vibriosis
Issue Date: Jun-2010
Publisher: Wiley-Blackwell
Citation: Sharifuzzaman SM & Austin B (2010) Kocuria SM1 controls vibriosis in rainbow trout (Oncorhynchus mykiss, Walbaum), Journal of Applied Microbiology, 108 (6), pp. 2162-2170.
Abstract: Aims: To develop probiotics for the control of vibriosis caused by Vibrio anguillarum and Vibrio ordalii in finfish. Methods and Results: Kocuria SM1, isolated from the digestive tract of rainbow trout, was administered orally to rainbow trout (Oncorhynchus mykiss) for 2 weeks at a dose equivalent to c. 108 cells per g of feed and then challenged intraperitoneally with V. anguillarum and V. ordalii. Use of SM1 led to a reduction in mortalities to 15–20% compared to 74–80% mortalities in the controls. SM1 stimulated both cellular and humoral immune responses in rainbow trout, by elevation of leucocytes (5·5 ± 0·8 × 106 ml−1 from 3·7 ± 0·8 × 106 ml−1), erythrocytes (1·2 ± 0·1 × 108 ml−1 from 0·8 ± 0·1 × 108 ml−1), protein (23 ± 4·4 mg ml−1 from 16 ± 1·3 mg ml−1), globulin (15·7 ± 0·2 mg ml−1 from 9·9 ± 0·1 mg ml−1) and albumin (7·3 ± 0·2 mg ml−1 from 6·1 ± 0·1 mg ml−1) levels, upregulation of respiratory burst (0·05 ± 0·01 from 0·02 ± 0·01), complement (56 ± 7·2 units ml−1 from 40 ± 8·0 units ml−1), lysozyme (920 ± 128·8 units ml−1 from 760 ± 115·3 units ml−1) and bacterial killing activities. Conclusions: Kocuria SM1 successfully controlled vibriosis in rainbow trout, and the mode of action reflected stimulation of the host innate immune system. Significance and Impact of the Study: Probiotics can contribute a significant role in fish disease control strategies, and their use
Type: Journal Article
URI: http://hdl.handle.net/1893/7374
DOI Link: http://dx.doi.org/10.1111/j.1365-2672.2009.04618.x
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Heriot-Watt University
Heriot-Watt University

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