Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/7372
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: PCR and molecular detection for differentiating Vibrio species
Authors: Sparagano, Olivier A E
Robertson, Peter
Purdom, Ian
McInnes, J
Li, Yun
Yu, Dehua
Du, Zong-Jun
Xu, Huaishu
Austin, Brian
Contact Email: brian.austin@stir.ac.uk
Keywords: vibriosis
16S PCR reactions
23S PCR reactions
Vibrio species
aquaculture
Issue Date: Oct-2002
Publisher: Wiley-Blackwell
Citation: Sparagano OAE, Robertson P, Purdom I, McInnes J, Li Y, Yu D, Du Z, Xu H & Austin B (2002) PCR and molecular detection for differentiating Vibrio species, Annals of the New York Academy of Sciences, 969 (1), pp. 60-65.
Abstract: Vibriosis is an economically important disease of fish, marine invertebrates (particularly penaeid shrimps), and large marine mammals and is responsible for high mortality rates in aquaculture worldwide. Some Vibrio species are also responsible for zoonoses, whereas others are relatively nonpathogenic. Using 16S- and 23S-based PCR reactions, we obtained species-specific patterns and a 470-bp band, respectively. DNA sequences obtained on the 23S rRNA gene allowed us to identify species-specific probes for Vibrio parahaemolyticus, V. alginolyticus, V. anguillarum and for a cluster of taxonomically related species: V. carchariae/harveyi/campbelii. A phylogenetic tree based on the 23S sequences confirmed previous results obtained by Western blotting.
Type: Journal Article
URI: http://hdl.handle.net/1893/7372
DOI Link: http://dx.doi.org/10.1111/j.1749-6632.2002.tb04351.x
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Heriot-Watt University
Heriot-Watt University
Heriot-Watt University
Heriot-Watt University
Ocean University of China
Ocean University of China
Shandong University
Ocean University of China
Heriot-Watt University

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