Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/7335
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Antigenic and cross-protection studies of biotype 1 and biotype 2 isolates of Yersinia ruckeri in rainbow trout, Oncorhynchus mykiss (Walbaum)
Authors: Tinsley, John W
Lyndon, Alastair R
Austin, Brian
Contact Email: brian.austin@stir.ac.uk
Keywords: Biotype 2
Enteric redmouth
IROMP
LPS
Nonmotile
Yersinia ruckeri
Issue Date: Jul-2011
Publisher: Wiley-Blackwell
Citation: Tinsley JW, Lyndon AR & Austin B (2011) Antigenic and cross-protection studies of biotype 1 and biotype 2 isolates of Yersinia ruckeri in rainbow trout, Oncorhynchus mykiss (Walbaum), Journal of Applied Microbiology, 111 (1), pp. 8- 16.
Abstract: Aims: The study investigated antigen characteristics of biotype (bt) 1 and bt 2 isolates of Yersinia ruckeri. Methods and Results: The cell surface characteristics of Y. ruckeri were compared for their antigenic characteristics using polyclonal antibodies that revealed that both biotypes had a homogenous whole-cell protein antigenic profile. Notable differences in the antigenic properties were observed in the lipopolysaccharide profile of both biotypes. Two iron-regulated outer membrane proteins (IROMP) of c. 90 and 100 kDa were shown to be major specific antigens. The results demonstrate for the first time differences in antigens between bt 1 and bt 2 isolates of serotype O1 isolates of Y. ruckeri. The protection induced in rainbow trout by a commercial monovalent, and bivalent inactivated vaccine was tested with the outcome that the ability of isolates to cause mortality in vaccinated fish varied with geographical location. In this context, vaccination studies suggested that the O antigen was the dominant immunogenic molecule involved in protection against the disease. Conclusions: The O antigen of Y. ruckeri was the dominant immunogenic molecule involved in the protection of rainbow trout against enteric redmouth disease. Significance and Impact of the Study: There are distinct phenotypic and antigenic differences in Y. ruckeri bt 1 and bt 2 with O antigen recognized as the dominant immunogenic molecule. The data have significance in explaining the lack of success of the earlier monovalent vaccine and demonstrate the effectiveness of the newer bivalent vaccine.
Type: Journal Article
URI: http://hdl.handle.net/1893/7335
DOI Link: http://dx.doi.org/10.1111/j.1365-2672.2011.05020.x
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Heriot-Watt University
Heriot-Watt University
Aquaculture

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