Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/7314
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Microbial diversity of intestinal contents and mucus in rainbow trout (Oncorhynchus mykiss)
Authors: Kim, Do-Hyung
Brunt, Jason
Austin, Brian
Contact Email: brian.austin@stir.ac.uk
Keywords: 16S rDNA
biodiversity
intestinal microflora
intestinal mucus
rainbow trout
Issue Date: Jun-2007
Publisher: Wiley-Blackwell
Citation: Kim D, Brunt J & Austin B (2007) Microbial diversity of intestinal contents and mucus in rainbow trout (Oncorhynchus mykiss), Journal of Applied Microbiology, 102 (6), pp. 1654-1664.
Abstract: Aims: The aim of this study was to understand the microbial community of intestinal contents and mucosal layer in the intestine of rainbow trout by means of culture-dependent conventional and independent molecular techniques. Methods and Results: Forty-one culturable microbial phylotypes, and 39 sequences from 16S rRNA and two from 18S rRNA genes, were retrieved. Aeromonadaceae, Enterobacteriaceae and Pseudomonadaceae representatives were the dominant cultured bacteria. Genomic DNA isolated from intestinal contents and mucus was used to generate 104 random clones, which were grouped into 32 phylotypes at 99% minimum similarity, most of which were affiliated with Proteobacteria (>70% of the total). However, unlike library C (intestinal contents), the phyla Bacteroidetes and Fusobacteria were not found in intestinal mucus (library M), indicating that the microbiota in the gut mucus was different from that of the intestinal contents. Twelve sequences were retrieved from denaturing gradient gel electrophoresis analysis, and dominant bands were mostly related to Clostridium. Conclusions: Many novel sequences that have not been previously recognized as part of the intestinal flora of rainbow trout were retrieved. Significance and Impact of the Study: The fish gut harbours a larger bacterial diversity than previously recognized, and the diversity of gut mucus is different from that of intestinal contents.
Type: Journal Article
URI: http://hdl.handle.net/1893/7314
DOI Link: http://dx.doi.org/10.1111/j.1365-2672.2006.03185.x
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Heriot-Watt University
Heriot-Watt University
Heriot-Watt University

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