Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/7303

Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: An inhibitor of bacterial quorum sensing reduces mortalities caused by vibriosis in rainbow trout (Oncorhynchus mykiss, Walbaum)
Authors: Rasch, Maria
Buch, Christiane
Austin, Brian
Slierendrecht, Wilhelmina J
Ekmann, Kim S
Larsen, Jens
Johansen, Charlotte
Riedel, Kathrin
Eberl, Leo
Givskov, Michael
Gram, Lone
Contact Email: brian.austin@stir.ac.uk
Keywords: Acylated homoserine lactones
Vibrio anguillarum
Quorum-sensing inhibitor
Furanone C-30
Issue Date: May-2004
Publisher: Elsevier
Citation: Rasch M, Buch C, Austin B, Slierendrecht WJ, Ekmann KS, Larsen J, Johansen C, Riedel K, Eberl L, Givskov M & Gram L (2004) An inhibitor of bacterial quorum sensing reduces mortalities caused by vibriosis in rainbow trout (Oncorhynchus mykiss, Walbaum), Systematic and Applied Microbiology, 27 (3), pp. 350-359.
Abstract: The fish pathogen Vibrio anguillarum produces quorum sensing signal molecules, N-acyl homoserine lactones (AHLs), which in several Gram-negative human and plant pathogenic bacteria regulate virulence factors. Expression of these factors can be blocked using specific quorum-sensing inhibitors (QSIs). The purpose of this study was to investigate the effect of a QSI, furanone C-30, on mortality of rainbow trout during challenge with V. anguillarum. Addition of 0.01 or 0.1 μM furanone C-30 to rainbow trout infected by cohabitation caused a significant reduction in accumulated mortality from 80-100% in challenge controls to 4-40% in treated groups. Furanone C-30 had no effect in an immersion challenge system, probably due to a very high water exchange and a rapid dilution of furanone C-30. Growth and survival of V. anguillarum were not affected by the concentrations of furanone C-30 used in the challenge experiments, thus avoiding selection for resistance. To elucidate the mechanism of disease control by furanone C-30, we determined its effect on the bacterial proteome, motility, and respiration. No effects were seen of furanone C-30 in any of these experiments. Although no cytotoxic effect on HeLa cells were observed, exposure to 1 micrometer (or higher) concentrations of furanone C-30 had detrimental effects on the rainbow trout. Our results indicate that QSIs can be used in non-antibiotic based control of fish diseases. However, they also underline the need for development of novel, less toxic QSI compounds and the need for understanding the exact mechanism(s) of action
Type: Journal Article
URI: http://hdl.handle.net/1893/7303
DOI Link: http://dx.doi.org/10.1078/0723-2020-00268
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Technical University of Denmark
Technical University of Denmark
Aquaculture
BioMar AS
BioMar AS
Royal Veterinary and Agricultural University, Denmark
Pantheco A/S, Denmark
Technische Universitat Munchen, Germany
Technical University of Denmark
Technical University of Denmark

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