Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/7302
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Efficacy of in-feed probiotics against Aeromonas bestiarum and Ichthyophthirius multifiliis skin infections in rainbow trout (Oncorhynchus mykiss, Walbaum)
Authors: Pieters, N
Brunt, Jason
Austin, Brian
Lyndon, Alastair R
Contact Email: brian.austin@stir.ac.uk
Keywords: Aeromonas bestiarum
fin rot
fish
Ich
Ichthyophthirius multifiliis
innate immunity
probiotics
white spot
Issue Date: Sep-2008
Publisher: Wiley-Blackwell
Citation: Pieters N, Brunt J, Austin B & Lyndon AR (2008) Efficacy of in-feed probiotics against Aeromonas bestiarum and Ichthyophthirius multifiliis skin infections in rainbow trout (Oncorhynchus mykiss, Walbaum), Journal of Applied Microbiology, 105 (3), pp. 723-732.
Abstract: Aims: The aim of this study was to assess the efficacy of in-feed probiotics as a preventive measure against skin infections caused by Aeromonas bestiarum and Ichthyophthirius multifiliis (Ich) in rainbow trout. Methods and Results: Fin rot was induced in fish by intradermal injection with 0·1 ml volumes containing 105 cells per ml A. bestiarum at the base of the dorsal fin. Ich infections resulted from immersion in Ich-contaminated water. Each probiotic was administered orally [108 cells per g feed for GC2 (Aeromonas sobria) and 1010 cells per g feed for BA211 (Brochothrix thermosphacta)] for 14 days. Results showed that, after challenge with A. bestiarum, probiotics GC2 and BA211 led to 76% and 88% survival, respectively, in contrast to 22% survival for controls. Fish fed with probiotic GC2 had 100% survival after challenge with Ich compared with 2% for probiotic BA211 and 0% for controls. Analysis of innate immune responses revealed that probiotic GC2 promoted higher phagocytic activity, whereas probiotic BA211 led to enhanced respiratory burst activity. Conclusion: Of the two probiotics examined, GC2 was more effective in protecting against both fin rot and Ich. Each probiotic appeared to stimulate different pathways within the innate immune system. Significance and Impact of the Study: This is the first demonstration that probiotics can protect fish against surface infections. Furthermore, this is the first time a probiotic has been shown to protect against a eucaryotic pathogen, namely I. multifiliis.
Type: Journal Article
URI: http://hdl.handle.net/1893/7302
DOI Link: http://dx.doi.org/10.1111/j.1365-2672.2008.03817.x
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Heriot-Watt University
Heriot-Watt University
Heriot-Watt University
Heriot-Watt University

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