Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/7216
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Pseudomonas sp M174 inhibits the fish pathogen Flavobacterium psychrophilum
Authors: Korkea-Aho, Tiina
Heikkinen, Jouni
Thompson, Kimberly
von, Wright Atte
Austin, Brian
Contact Email: brian.austin@stir.ac.uk
Keywords: antagonism
biocontrol
Flavobacterium psychrophilum
growth inhibition
probiotic
Pseudomonas sp.
Issue Date: Aug-2011
Publisher: Wiley-Blackwell
Citation: Korkea-Aho T, Heikkinen J, Thompson K, von Wright A & Austin B (2011) Pseudomonas sp M174 inhibits the fish pathogen Flavobacterium psychrophilum, Journal of Applied Microbiology, 111 (2), pp. 266-277.
Abstract: Aims: To study the antagonic affect of probiotic Pseudomonas M174 on the fish pathogen Flavobacterium psychrophilum. Methods and Results: The ability of Pseudomonas M174 to inhibit the growth of Fl. psychrophilum was examined in iron-sufficient and -deficient media. Possible siderophore production was also investigated. Antagonistic activity was confirmed in disease challenge experiments using a rainbow trout (Oncorhynchus mykiss) model. Adhesion of Pseudomonas M174 to fish surfaces and its ability to stimulate innate immunity was also investigated in vivo. Pseudomonas M174 antagonized Fl. psychrophilum and produced siderophores in vitro. In challenge experiments with Fl. psychrophilum, fish fed with Pseudomonas M174 had lower levels of mortalities than the controls. It was possible to find Pseudomonas M174 in the intestinal content of these fish after feeding and bathing with the probiotic, but probiotic was obtained from the gills only after feeding. Respiratory burst activity was also found to be enhanced in the M174 fed fish. Conclusions: These results suggest that M174 is a potential probiotic against Fl. psychrophilum and has several modes of action. Significance and Impact of the Study: Probiotics are a promising alternative to the use of antibiotics in aquaculture and could be a more sustainable disease control method.
Type: Journal Article
URI: http://hdl.handle.net/1893/7216
DOI Link: http://dx.doi.org/10.1111/j.1365-2672.2011.05044.x
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Aquaculture
University of Eastern Finland
Aquaculture
University of Eastern Finland
Aquaculture

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