Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/7213
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Identification and pathogenicity to rainbow trout, Oncorhynchus mykiss (Walbaum), of some aeromonads
Authors: Orozova, Petya
Barker, Margaret
Austin, Dawn A
Austin, Brian
Contact Email: brian.austin@stir.ac.uk
Keywords: Aeromonas spp
fish pathogens
identity
pathogenicity
rainbow trout
Issue Date: Oct-2009
Publisher: Wiley-Blackwell
Citation: Orozova P, Barker M, Austin DA & Austin B (2009) Identification and pathogenicity to rainbow trout, Oncorhynchus mykiss (Walbaum), of some aeromonads, Journal of Fish Diseases, 32 (10), pp. 865-871.
Abstract: Twelve strains of fish pathogenic aeromonads were identified by 16S rRNA sequencing as Aeromonas bestiarum, A. hydrophila, A. hydrophila subsp. dhakensis, A. salmonicida subsp. salmonicida, A. sobria biovar sobria and A. veronii biovar sobria. Following intramuscular injection, A. hydrophila subsp. dhakensis caused dark liquefying, raised furuncle-like lesions in rainbow trout within 48 h. Extracellular products of all cultures contained gelatinase and lecithinase, and most revealed lipase. Congo red absorption and siderophore production was recorded, but not so the suicide phenomenon or slime production. Sodium dodecyl sulphate polyacrylamide gel electrophoresis profile of the outer membrane proteins (OMP) revealed 10–25 bands, of which major bands were seen in the region of 32.5–47.5 and 62–83 kDa. Marked heterogenicity of the OMP and whole cell protein (WCP) profiles within and among the species was observed. Polypeptides of 83–173 kDa were detected in the WCP profile of the cultures, but they were not expressed in OMP fractions.
Type: Journal Article
URI: http://hdl.handle.net/1893/7213
DOI Link: http://dx.doi.org/10.1111/j.1365-2761.2009.01065.x
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Bulgarian Academy of Sciences
Heriot-Watt University
Heriot-Watt University
Heriot-Watt University

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