Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/3731
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dc.contributor.authorMinghetti, Matteoen_UK
dc.contributor.authorLeaver, Michaelen_UK
dc.contributor.authorTaggart, Johnen_UK
dc.contributor.authorCasadei, Elisaen_UK
dc.contributor.authorAuslander, Meiraven_UK
dc.contributor.authorTom, Mosheen_UK
dc.contributor.authorGeorge, Stephenen_UK
dc.date.accessioned2012-07-10T23:37:30Z-
dc.date.available2012-07-10T23:37:30Z-
dc.date.issued2011-08en_UK
dc.identifier.urihttp://hdl.handle.net/1893/3731-
dc.description.abstractCopper transporting ATPase, ATP7A, is an ATP dependent copper pump present in all vertebrates, critical for the maintenance of intracellular and whole body copper homeostasis. Effects of copper treatment on ATP7A gene expression in fibroblast cells (SAF1) of the sea bream (Sparus aurata) were investigated by qRT-PCR and by a medium density microarray from a closely related species, striped sea bream (Lithognathus mormyrus). To discriminate between the effects of Cu and other metals, SAF1 cells were exposed to sub-toxic levels of Cu, Zn and Cd. Expression of Cu homeostasis genes copper transporter 1 (CTR1), Cu ATPase (ATP7A), Cu chaperone (ATOX1) and metallothionein (MT) together with the oxidative stress markers glutathione reductase (GR) and Cu/Zn superoxide dismutase (CuZn/SOD) were measured 0, 4 and 24 hours post-exposure by qRT-PCR. Microarray was conducted on samples from 4 hours post Cu exposure. Cu, Zn and Cd increased MT and GR mRNA levels, while only Cu increased ATP7A mRNA levels. Microarray results confirmed the effects of Cu on ATP7A and MT and in addition showed changes in the expression of genes involved in protein transport and secretion. Results suggest that ATP7A may be regulated at the transcriptional level directly by Cu and by a mechanism that is different from that exerteted by metals on MT genes.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationMinghetti M, Leaver M, Taggart J, Casadei E, Auslander M, Tom M & George S (2011) Copper induces Cu-ATPase ATP7A mRNA in a fish cell line, SAF1. Comparative Biochemistry and Physiology - Part C: Toxicology and Pharmacology, 154 (2), pp. 93-99. http://www.sciencedirect.com/science/article/pii/S153204561100055X; https://doi.org/10.1016/j.cbpc.2011.03.010en_UK
dc.rightsPublished in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology by Elsevier. This is the peer reviewed version of this article. NOTICE: this is the author’s version of a work that was accepted for publication in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, VOL 154, ISSUE 2, (August 2011). DOI: 10.1016/j.cbpc.2011.03.010.en_UK
dc.subjectCopperen_UK
dc.subjectMetalsen_UK
dc.subjectATP7Aen_UK
dc.subjectMTen_UK
dc.subjectMicroarrayen_UK
dc.subjectqRT-PCRen_UK
dc.subjectFishen_UK
dc.subjectSparus aurataen_UK
dc.subjectFishes Feeding and feedsen_UK
dc.subjectAquacultureen_UK
dc.subjectFishes Nutritionen_UK
dc.titleCopper induces Cu-ATPase ATP7A mRNA in a fish cell line, SAF1en_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1016/j.cbpc.2011.03.010en_UK
dc.citation.jtitleComparative Biochemistry and Physiology - Part C: Toxicology and Pharmacologyen_UK
dc.citation.issn1532-0456en_UK
dc.citation.volume154en_UK
dc.citation.issue2en_UK
dc.citation.spage93en_UK
dc.citation.epage99en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S153204561100055Xen_UK
dc.author.emailm.j.leaver@stir.ac.uken_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
dc.contributor.affiliationIsrael Oceanographic and Limnological Researchen_UK
dc.contributor.affiliationIsrael Oceanographic and Limnological Researchen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isiWOS:000292179100004en_UK
dc.identifier.scopusid2-s2.0-79957741558en_UK
dc.identifier.wtid792142en_UK
dc.contributor.orcid0000-0002-3155-0844en_UK
dc.contributor.orcid0000-0002-3843-9663en_UK
dcterms.dateAccepted2011-08-31en_UK
dc.date.filedepositdate2012-03-20en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorMinghetti, Matteo|en_UK
local.rioxx.authorLeaver, Michael|0000-0002-3155-0844en_UK
local.rioxx.authorTaggart, John|0000-0002-3843-9663en_UK
local.rioxx.authorCasadei, Elisa|en_UK
local.rioxx.authorAuslander, Meirav|en_UK
local.rioxx.authorTom, Moshe|en_UK
local.rioxx.authorGeorge, Stephen|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2012-03-20en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/all-rights-reserved|2012-03-20|en_UK
local.rioxx.filenameMinghettiCBPC2011storre.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source1532-0456en_UK
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