Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/3570
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Development of a fish cell culture model to investigate the impact of fish oil replacement on lipid peroxidation
Authors: Gregory, Melissa K
King, Hamish W
Bain, Peter A
Gibson, Robert A
Tocher, Douglas R
Schuller, Kathryn A
Contact Email: drt1@stir.ac.uk
Keywords: Aquaculture
beta-oxidation
cell culture
fish oil
lipid peroxidation
phospholipids
polyunsaturated fatty acids
biosynthesis
Issue Date: Aug-2011
Publisher: Springer Verlag
Citation: Gregory MK, King HW, Bain PA, Gibson RA, Tocher DR & Schuller KA (2011) Development of a fish cell culture model to investigate the impact of fish oil replacement on lipid peroxidation, Lipids, 46 (8), pp. 753-764.
Abstract: Fish oils are rich in omega-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA), predominantly 20:5n-3 and 22:6n-3, whereas vegetable oils contain abundant C18-PUFA, predominantly 18:3n-3 or 18:2n-6. We hypothesized that replacement of fish oils with vegetable oils would increase the oxidative stability of fish lipids. Here we have used the FHM cell line to test this hypothesis. The FHM cells were readily able to synthesize 20:5n-3 and 24:6n-3 from 18:3n-3 but 22:6n-3 synthesis was negligible. Also, they were readily able to synthesize 20:3n-6 from 18:2n-6 but 20:4n-6 synthesis was negligible. Mitochondrial β-oxidation was greatest for 18:3n-3 and 20:5n-3 and the rates for 16:0, 18:2n-6, 22:6n-3 and 18:1n-9 were significantly lower. Fatty acid incorporation was predominantly into phospholipids (79-97%) with very little incorporation into neutral lipids. Increasing the fatty acid concentration in the growth medium substantially increased the concentrations of 18:3n-3 and 18:2n-6 in the cell phospholipids but this was not the case for 20:5n-3 or 22:6n-3. When they were subjected to oxidative stress, the FHM cells supplemented with either 20:5n-3 or 22:6n-3 (as compared with 18:3n-3 or saturated fatty acids) exhibited significantly higher levels of thiobarbituric reactive substances (TBARS) indicating higher levels of lipid peroxidation. The results are discussed in relation to the effects of fatty acid unsaturation on the oxidative stability of cellular lipids and the implications for sustainable aquaculture.
Type: Journal Article
URI: http://hdl.handle.net/1893/3570
DOI Link: http://dx.doi.org/10.1007/s11745-011-3558-9
Rights: Published in Lipids by Springer Verlag. http://www.springerlink.com/content/ft8q288332441018/. The original publication is available at www.springerlink.com
Affiliation: Flinders University
Flinders University
Flinders University
University of Adelaide
Aquaculture
Flinders University

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