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http://hdl.handle.net/1893/33381| Appears in Collections: | Aquaculture Journal Articles |
| Peer Review Status: | Refereed |
| Title: | A high-density linkage map and sex-linked markers for the Amazon Tambaqui Colossoma macropomum |
| Author(s): | Varela, Eduardo Sousa Bekaert, Michaël Ganeco-Kirschnik, Luciana Nakaghi Torati, Lucas Simon Shiotsuki, Luciana de Almeida, Fernanda Loureiro Villela, Luciana Cristine Vasques Rezende, Fabrício Pereira da Silva Barroso, Aurisan de Freitas, Luiz Eduardo Lima Taggart, John Bernard Migaud, Herve |
| Contact Email: | michael.bekaert@stir.ac.uk |
| Keywords: | Genetics Biotechnology |
| Issue Date: | Dec-2021 |
| Date Deposited: | 6-Oct-2021 |
| Citation: | Varela ES, Bekaert M, Ganeco-Kirschnik LN, Torati LS, Shiotsuki L, de Almeida FL, Villela LCV, Rezende FP, da Silva Barroso A, de Freitas LEL, Taggart JB & Migaud H (2021) A high-density linkage map and sex-linked markers for the Amazon Tambaqui Colossoma macropomum. BMC Genomics, 22 (1), Art. No.: 709. https://doi.org/10.1186/s12864-021-08037-8 |
| Abstract: | Tambaqui (\textit{Colossoma macropomum}, Cuvier, 1818) is the most economically important native freshwater fish species in Brazil. It can reach a total length of over 1~m and a weight of over 40~kg. The species displays a clear sex dimorphism in growth performance with females reaching larger sizes at harvest. In aquaculture, the production of monosex populations in selective breeding programmes has been therefore identified as a key priority. In the present study, a genetic linkage map was generated by double digest restriction-site associated DNA (ddRAD) sequencing from 248 individuals sampled from two F1 families. The map was constructed using 14,805 informative SNPs and spanned 27 linkage groups. From this, the tambaqui draft genome was improved, by ordering the scaffolds into chromosomes, and sex-linked markers were identified. A total of 235 markers on linkage group 26 showed a significant association with the phenotypic sex supporting an XX/XY sex determination system in the species. The four most informative sex-linked markers were validated on another 206 sexed individuals demonstrating an accuracy in predicting sex ranging from 90.0\% to 96.7\%. The genetic mapping and novel sex-linked DNA markers identified and validated offer new tools for rapid progeny sexing, thus supporting the development of monosex female production in the industry while also supporting breeding programs of the species. |
| DOI Link: | 10.1186/s12864-021-08037-8 |
| Rights: | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
| Licence URL(s): | http://creativecommons.org/licenses/by/4.0/ |
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| File | Description | Size | Format | |
|---|---|---|---|---|
| s12864-021-08037-8.pdf | Fulltext - Published Version | 1.33 MB | Adobe PDF | View/Open |
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